Search Results - "KANEKO, Takehito"

Simple sperm preservation by freeze-drying for conserving animal strains by Kaneko, Takehito

“…Freeze-drying spermatozoa is the ultimate method for the maintenance of animal strains, in that the gametes can be preserved for a long time in a refrigerator…”
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ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes by Yoshimi, Kazuto, Kunihiro, Yayoi, Kaneko, Takehito, Nagahora, Hitoshi, Voigt, Birger, Mashimo, Tomoji

“…The CRISPR-Cas system is a powerful tool for generating genetically modified animals; however, targeted knock-in (KI) via homologous recombination remains…”
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Simple Genome Editing of Rodent Intact Embryos by Electroporation by Kaneko, Takehito, Mashimo, Tomoji

“…The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) system is a powerful tool for genome editing in animals…”
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Treatment with MG132 prevents spontaneous activation of rat oocyte in culture and promotes embryonic development after intracytoplasmic sperm injection by Nakagawa, Yuki, Kaneko, Takehito

“…Intracytoplasmic sperm injection (ICSI) is an effective reproductive technique for obtaining rat offspring using preserved sperm with low or no motility…”
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Reproductive technologies for the generation and maintenance of valuable animal strains by KANEKO, Takehito

“…Many types of mutant and genetically engineered strains have been produced in various animal species. Their numbers have dramatically increased in recent…”
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Improvement of genome editing by electroporation using embryos artificially removed cumulus cells in the oviducts by Kaneko, Takehito, Tanaka, Shungo

“…Many genome-edited animals have been produced using the CRISPR/Cas system. Genome-edited strains were produced by introducing nucleases into pronuclear stage…”
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Production of genome-edited mice by visualization of nucleases introduced into the embryos using electroporation by WAKE, Yui, KANEKO, Takehito

“…Genome editing technology contributes to the quick and highly efficient production of genetically engineered animals. These animals are helpful in clarifying…”
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Vitrification of canine ovarian tissues with polyvinylpyrrolidone preserves the survival and developmental capacity of primordial follicles by Fujihara, Mayako, Kaneko, Takehito, Inoue-Murayama, Miho

“…Ovarian tissue cryopreservation combined with immature follicle development can preserve female fertility in wildlife, regardless of age or reproductive…”
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Tolerance to vitrification of rat embryos at various developmental stages by Taketsuru, Hiroaki, Kaneko, Takehito

“…Numerous genetically engineered rat strains have been produced via genome editing. Although freezing of embryos is helpful for the production and storage of…”
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Successful pseudopregnancy of rats by short period artificial stimulation using sonic vibration by Endo, Marina, Tsunoda, Shigemi, Tawara, Hirosuke, Abe, Hisayuki, Kaneko, Takehito

“…Psuedopregnancy for embryo transfer (ET) is usually induced in rats by mating with vasectomized males. Previously, we successfully induced pseudopregnancy…”
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Simple induction of pseudopregnancy by artificial stimulation using a sonic vibration in rats by Kaneko, Takehito, Endo, Marina, Tsunoda, Shigemi, Nakagawa, Yuki, Abe, Hisayuki

“…Embryo transfer has been used as one of the essential reproductive technologies for production of new strains and maintenance of genetic resources in animals…”
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Creating knockout and knockin rodents using engineered endonucleases via direct embryo injection by Kaneko, Takehito, Mashimo, Tomoji

“…Genetically engineered rodents have been generated worldwide for biomedical research. Recently, gene targeting techniques have been developed by using…”
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Successful induction of pseudopregnancy using sonic vibration in mice by Wake, Yui, Endo, Marina, Tsunoda, Shigemi, Tawara, Hirosuke, Abe, Hisayuki, Nakagawa, Yuki, Kaneko, Takehito

“…Embryo transfer (ET) is an essential reproductive technology for the production of new animal strains and maintenance of genetic resources. We developed a…”
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Brca1L63X/+ rat is a novel model of human BRCA1 deficiency displaying susceptibility to radiation‐induced mammary cancer by Nakamura, Yuzuki, Kubota, Jo, Nishimura, Yukiko, Nagata, Kento, Nishimura, Mayumi, Daino, Kazuhiro, Ishikawa, Atsuko, Kaneko, Takehito, Mashimo, Tomoji, Kokubo, Toshiaki, Takabatake, Masaru, Inoue, Kazumasa, Fukushi, Masahiro, Arai, Masami, Saito, Mitsue, Shimada, Yoshiya, Kakinuma, Shizuko, Imaoka, Tatsuhiko

“…Women who are heterozygous for deleterious BRCA1 germline mutations harbor a high risk of hereditary breast cancer. Previous Brca1‐heterozygous animal models…”
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Amount of Cas9 protein introduced into mouse embryos via electroporation affects the genome-editing rate by WAKE, Yui, VAKULSKAS, Christopher A., GLENN, Steve E., KANEKO, Takehito

“…Genetically engineered animals can be produced quickly using genome editing technology. A new electroporation technique, technique for animal knockout system…”
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Effects of the Prdx2 depletion on blood pressure and life span in spontaneously hypertensive rats by Mahal, Zinat, Fujikawa, Koichi, Matsuo, Hiroyuki, Zahid, Hasan M, Koike, Masamichi, Misumi, Masaki, Kaneko, Takehito, Mashimo, Tomoji, Ohara, Hiroki, Nabika, Toru

“…Oxidative stress is involved in the pathogenesis of hypertension and hypertensive organ damage. Our previous study suggested that stroke-prone spontaneously…”
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Efficient collection and cryopreservation of embryos in F344 strain inbred rats by Taketsuru, Hiroaki, Kaneko, Takehito

“…In rats, it is now possible to produce genetically engineered strains, not only as transgenic animals but also using gene knockout techniques. Reproductive…”
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Successful long-term preservation of rat sperm by freeze-drying by Kaneko, Takehito, Serikawa, Tadao

“…Freeze-drying sperm has been developed as a new preservation method where liquid nitrogen is no longer necessary. An advantage of freeze-drying sperm is that…”
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LOX-1 (Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1) Deletion Has Protective Effects on Stroke in the Genetic Background of Stroke-Prone Spontaneously Hypertensive Rat by Liang, Yi-Qiang, Kakino, Akemi, Matsuzaka, Yasunari, Mashimo, Tomoji, Isono, Masato, Akamatsu, Tomohisa, Shimizu, Hana, Tajima, Michiko, Kaneko, Takehito, Li, Lei, Takeuchi, Fumihiko, Sawamura, Tatsuya, Kato, Norihiro

“…BACKGROUND AND PURPOSE—oxLDL (oxidized low-density lipoprotein) has been known for its potential to induce endothelial dysfunction and used as a major…”
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Sperm preservation by freeze-drying for the conservation of wild animals by Kaneko, Takehito, Ito, Hideyuki, Sakamoto, Hidefusa, Onuma, Manabu, Inoue-Murayama, Miho

“…Sperm preservation is a useful technique for the maintenance of biological resources in experimental and domestic animals, and in wild animals. A new…”
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