Search Results - "KALOUSEK, F"

cysteine endopeptidase with a C-terminal KDEL motif isolated from castor bean endosperm is a marker enzyme for the ricinosome, a putative lytic compartment by Schmid, M, Simpson, D, Kalousek, F, Gietl, C

“…A papain-type cysteine endopeptidase with a molecular mass of 35 kDa for the mature enzyme, was purified from germinating castor bean (Ricinus communis L.)…”
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Rat liver mitochondrial intermediate peptidase (MIP): purification and initial characterization by Kalousek, F., Isaya, G., Rosenberg, L.E.

“…A number of nuclearly encoded mitochondrial protein precursors that are transported into the matrix and inner membrane are cleaved in two sequential steps by…”
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Rat liver mitochondrial processing peptidase. Both alpha- and beta-subunits are required for activity by SAAVEDRA-ALANIS, V. M, RYSAVY, P, ROSENBERG, L. E, KALOUSEK, F

“…Most nuclearly encoded mitochondrial proteins are synthesized with an amino-terminal leader peptide that is cleaved by the mitochondrial processing peptidase…”
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GroEL, GroES, and ATP-dependent folding and spontaneous assembly of ornithine transcarbamylase by XIANXIAN ZHENG, ROSENBERG, L. E, KALOUSEK, F, FENTON, W. A

“…When purified rat liver ornithine transcarbamylase (OTC), a trimer of 36 kDa subunits, was denatured in 6 M guanidine hydrochloride and then diluted…”
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A cysteine endopeptidase isolated from castor bean endosperm microbodies processes the glyoxysomal malate dehydrogenase precursor protein by Gietl, C. (Technical University of Munich, Munich, Germany.), Wimmer, B, Adamex, J, Kalousek, F

“…A plant cysteine endopeptidase with a molecular mass of 35 kD was purified from microbodies of germinating castor bean (Ricinus communis) endosperm by virtue…”
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Cleavage of Precursors by the Mitochondrial Processing Peptidase Requires a Compatible Mature Protein or an Intermediate Octapeptide by Isaya, Grazia, Kalousek, Frantisek, Fenton, Wayne A., Rosenberg, Leon E.

“…Many precursors of mitochondrial proteins are processed in two successive steps by independent matrix peptidases (MPP and MIP), whereas others are cleaved in a…”
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Two Mitochondrial Matrix Proteases Act Sequentially in the Processing of Mammalian Matrix Enzymes by Kalousek, Frantisek, Hendrick, Joseph P., Rosenberg, Leon E.

“…The imported precursors of the mammalian matrix enzymes malate dehydrogenase [(S)-malate:NAD+ oxidoreductase, EC 1.1.1.37] and ornithine transcarbamylase…”
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The General Mitochondrial Matrix Processing Protease from Rat Liver: Structural Characterization of the Catalytic Subunit by Kleiber, Jorg, Kalousek, Frantisek, Swaroop, Manju, Rosenberg, Leon E.

“…A critical step in the import of nuclear-encoded precursor proteins into mitochondria involves proteolytic cleavage of their amino-terminal leader peptides by…”
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A leader peptide is sufficient to direct mitochondrial import of a chimeric protein by Horwich, A.L., Kalousek, F., Mellman, I., Rosenberg, L.E.

“…Most mitochondrial proteins are encoded in the nucleus and synthesized in the cytoplasm as larger precursors containing NH2‐terminal ‘leader’ peptides. To test…”
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PCR method for generating multiple mutations at adjacent sites by ADAMEC, J, KALOUSEK, F

“…Procedures to introduce point mutations, restriction sites and insert or delete DNA fragments are very important tools to study protein function. We describe…”
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Amino-terminal octapeptides function as recognition signals for the mitochondrial intermediate peptidase by ISAYA, G, KALOUSEK, F, ROSENBERG, L. E

“…We have shown previously that cleavage of a number of precursors by the mitochondrial processing peptidase (MPP) requires an intermediate octapeptide…”
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Sequence Analysis of Rat Mitochondrial Intermediate Peptidase: Similarity to Zinc Metallopeptidases and to a Putative Yeast Homologue by Isaya, Grazia, Kalousek, Frantisek, Rosenberg, Leon E.

“…Proteolytic removal of amino-terminal octapeptides from mitochondrial intermediate proteins is a required step for a subgroup of nuclear-encoded mitochondrial…”
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The β Subunit of the Mitochondrial Processing Peptidase From Rat Liver: Cloning and Sequencing of a cDNA and Comparison With a Proposed Family of Metallopeptidases by Paces, Vaclav, Rosenberg, Leon E., Fenton, Wayne A., Kalousek, Frantisek

“…Most nuclearly encoded mitochondrial proteins are synthesized with amino-terminal leader peptides that are removed by the mitochondrial processing peptidase…”
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Arginine in the Leader Peptide is Required for Both Import and Proteolytic Cleavage of a Mitochondrial Precursor by Horwich, A. L., Kalousek, F., Rosenberg, L. E.

“…Most mitochondrial proteins are encoded in the nucleus and translated in the cytoplasm as larger precursors containing NH2-terminal ``leader'' peptides, which…”
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Isolation and characterization of propionyl-CoA carboxylase from normal human liver. Evidence for a protomeric tetramer of nonidentical subunits by Kalousek, F, Darigo, M D, Rosenberg, L E

“…We have purified propionyl-CoA carboxylase from normal, postmortem human liver to homogeneity. The isolation procedure, which provided an approximately…”
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Different Structures in the Amino-Terminal Domain of the Ornithine Transcarbamylase Leader Peptide are Involved in Mitochondrial Import and Carboxyl-Terminal Cleavage by Kraus, Jan P., Novotný, Jiří, Kalousek, František, Swaroop, Manju, Rosenberg, Leon E.

“…The cytoplasmic precursor of mitochondrial ornithine transcarbamylase (carbamoyl-phosphate:L-ornithine carbamoyltransferase, EC 2.1.3.2) contains an…”
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Coding Sequence of the Precursor of the β Subunit of Rat Propionyl-CoA Carboxylase by Kraus, Jan P., Firgaira, Frank, Novotný, Jiří, Kalousek, František, Williams, Kenneth R., Williamson, Cynthia, Ohura, Toshihiro, Rosenberg, Leon E.

“…A cDNA encoding the cytoplasmic precursor of the β subunit of the mitochondrial enzyme propionyl-CoA carboxylase (EC 6.4.1.3) was cloned and sequenced. The DNA…”
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Import of Rat Ornithine Transcarbamylase Precursor into Mitochondria: Two-Step Processing of the Leader Peptide by Sztul, Elizabeth S., Hendrick, Joseph P., Kraus, Jan P., Wall, Doris, Kalousek, Frantisek, Rosenberg, Leon E.

“…The mitochondrial matrix enzyme ornithine transcarbamylase (OTC) is synthesized on cytoplasmic polyribosomes as a precursor (pOTC) with an NH2-terminal…”
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Mitochondrial import and processing of mutant human ornithine transcarbamylase precursors in cultured cells by ISAYA, G, FENTON, W. A, HENDRICK, J. P, FURTAK, K, KALOUSEK, F, ROSENBERG, L. E

“…Article Usage Stats Services MCB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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Newly processed ornithine transcarbamylase subunits are assembled to trimers in rat liver mitochondria by Kalousek, F, Orsulak, M D, Rosenberg, L E

“…We have characterized further the biogenesis in vitro of ornithine transcarbamylase, a homotrimeric mitochondrial matrix enzyme synthesized in the cytoplasm as…”
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