Search Results - "KAARSHOLM, N. C"

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  1. 1

    Properties of Small Molecules Affecting Insulin Receptor Function by Schlein, Morten, Ludvigsen, Svend, Olsen, Helle B, Andersen, Asser S, Danielsen, Gillian M, Kaarsholm, Niels C

    Published in Biochemistry (Easton) (13-11-2001)
    “…Small molecules with insulin mimetic effects and oral availability are of interest for potential substitution of insulin injections in the treatment of…”
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  2. 2

    Spectroscopic signatures of the T to R conformational transition in the insulin hexamer by Roy, M, Brader, M L, Lee, R W, Kaarsholm, N C, Hansen, J F, Dunn, M F

    Published in The Journal of biological chemistry (15-11-1989)
    “…The cobalt(II)-substituted human insulin hexamer has been shown to undergo the phenol-induced T6 to R6 structural transition in solution. The accompanying…”
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  3. 3

    Ligand-induced conformation change in folate-binding protein by KAARSHOLM, N. C, KOLSTRUP, A.-M, DANIELSEN, S. E, HOLM, J, HANSEN, S. I

    Published in Biochemical journal (15-06-1993)
    “…C.d. and fluorescence spectroscopy have been used to investigate the effect of ligand binding on the structure and stability of folate-binding protein (FBP)…”
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  4. 4

    Engineering stability of the insulin monomer fold with application to structure-activity relationships by KAARSHOLM, N. C, NORRIS, K, JØRGENSEN, R. J, MIKKELSEN, J, LUDVIGSEN, S, OLSEN, O. H, SØRENSEN, A. R, HAVELUND, S

    Published in Biochemistry (Easton) (12-10-1993)
    “…To evaluate the possible relationship between biological activity and structural stability in selected regions of the insulin molecule, we have analyzed the…”
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  5. 5

    Allosteric transition of the insulin hexamer is modulated by homotropic and heterotropic interactions by Choi, Wonjae E., Brader, Mark L., Aguilar, Valentin, Kaarsholm, Niels C., Dunn, Michael F.

    Published in Biochemistry (Easton) (02-11-1993)
    “…The allosteric behavior of the Co(II)-substituted insulin hexamer has been investigated using electronic spectroscopy to study the binding of different…”
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  6. 6

    Ionization behavior of native and mutant insulins: pK perturbation of B13-Glu in aggregated species by Kaarsholm, N C, Havelund, S, Hougaard, P

    Published in Archives of biochemistry and biophysics (01-12-1990)
    “…Upscale titration from pH 2.5 to 11.2 is used as a means for probing solvent accessibility of ionizing groups in zinc-free preparations of native and mutant…”
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  7. 7

    Characterization of the r-state insulin hexamer and its derivatives. The hexamer is stabilized by heterotropic ligand binding interactions by Brader, Mark L, Kaarsholm, Niels C, Lee, Robert W. K, Dunn, Michael F

    Published in Biochemistry (Easton) (09-07-1991)
    “…1H NMR and UV-visible electronic absorption studies have been performed to investigate the effects of anions and cyclic organic molecules on the…”
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  8. 8

    Engineering-enhanced Protein Secretory Expression in Yeast with Application to Insulin by Kjeldsen, Thomas, Ludvigsen, Svend, Diers, Ivan, Balschmidt, Per, Sørensen, Anders R., Kaarsholm, Niels C.

    Published in The Journal of biological chemistry (24-05-2002)
    “…Adaptation to efficient heterologous expression is a prerequisite for recombinant proteins to fulfill their clinical and biotechnological potential. We…”
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  9. 9

    The pro region required for folding of carboxypeptidase Y is a partially folded domain with little regular structural core by Soerensen, Poul, Winther, Jakob R, Kaarsholm, Niels C, Poulsen, Flemming M

    Published in Biochemistry (Easton) (16-11-1993)
    “…The pro region of carboxypeptidase Y (CPY) from yeast is necessary for the correct folding of the enzyme [Winther, J.R., and Sorensen P. (1991) Proc. Natl…”
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  10. 10

    A structural switch in a mutant insulin exposes key residues for receptor binding by Ludvigsen, S, Olsen, H B, Kaarsholm, N C

    Published in Journal of molecular biology (29-05-1998)
    “…Despite years of effort to clarify the structural basis of insulin receptor binding no clear consensus has emerged. It is generally believed that insulin…”
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  11. 11

    Solution Structure of an Engineered Insulin Monomer at Neutral pH by Olsen, Helle B, Ludvigsen, Svend, Kaarsholm, Niels C

    Published in Biochemistry (Easton) (09-07-1996)
    “…Insulin circulates in the bloodstream and binds to its specific cell-surface receptor as a 5808 Da monomeric species. However, studies of the monomer structure…”
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  12. 12

    Structural Effects of Protein Lipidation as Revealed by LysB29-myristoyl, des(B30) Insulin by Olsen, Helle B, Kaarsholm, Niels C

    Published in Biochemistry (Easton) (03-10-2000)
    “…Intracellular proteins are frequently modified by covalent addition of lipid moieties such as myristate. Although a functional role of protein lipidation is…”
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  13. 13

    Studies of the association and conformational properties of metal-free insulin in alkaline sodium chloride solutions by one- and two-dimensional 1H NMR by KADIMA, W, ROY, M, LEE, R. W.-K, KAARSHOLM, N. C, DUNN, M. F

    Published in The Journal of biological chemistry (05-05-1992)
    “…One- and two-dimensional 1H NMR spectroscopy have been employed to probe the association and subsequent conformational changes of metal-free insulin in sodium…”
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  14. 14

    The R-state proinsulin and insulin hexamers mimic the carbonic anhydrase active site by BRADER, M. LO, KAARSHILM, N. C, DUNN, M. F

    Published in The Journal of biological chemistry (15-09-1990)
    “…The cobalt(II)-substituted proinsulin and insulin hexamers have been studied in solution via electronic absorption spectroscopy. Hexameric proinsulin is shown…”
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  15. 15

    Mechanisms of Stabilization of the Insulin Hexamer through Allosteric Ligand Interactions by Rahuel-Clermont, Sophie, French, Cherie A, Kaarsholm, Niels C, Dunn, Michael F

    Published in Biochemistry (Easton) (13-05-1997)
    “…The insulin hexamer is an allosteric protein capable of undergoing transitions between three conformational states:  T6, T3R3, and R6. These transitions are…”
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  16. 16

    The relationship between insulin bioactivity and structure in the NH2-terminal A-chain helix by Olsen, H B, Ludvigsen, S, Kaarsholm, N C

    Published in Journal of molecular biology (27-11-1998)
    “…Studies of naturally occuring and chemically modified insulins have established that the NH2-terminal helix of the A-chain is important in conferring affinity…”
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  17. 17

    High-resolution structure of an engineered biologically potent insulin monomer, B16 Tyr-->His, as determined by nuclear magnetic resonance spectroscopy by Ludvigsen, S, Roy, M, Thøgersen, H, Kaarsholm, N C

    Published in Biochemistry (Easton) (05-07-1994)
    “…Site-directed mutagenesis is used in conjunction with 1H nuclear magnetic resonance (NMR) and circular dichroism (CD) spectroscopy in order to find an insulin…”
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  18. 18

    Subsite differences between the active centres of papaya peptidase A and papain as revealed by affinity chromatography. Purification of papaya peptidase A by ionic-strength-dependent affinity adsorption on an immobilized peptide inhibitor of papain by Schack, P, Kaarsholm, N C

    Published in Biochemical journal (01-05-1984)
    “…An affinity column consisting of the specific peptide inhibitor of papain, Gly-Gly (O-benzyl)Tyr-Arg, attached to Sepharose was found to bind the active thiol…”
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