Investigation of BK Virus by Real-Time Polymerase Chain Reaction in Patients With Allogeneic Bone Marrow Transplantation
BK virus (BKV) is a common human polyomavirus and causes latent infection. Especially in immunosuppressive patients, early diagnosis and treatment are very important in reducing the high mortality rate. In this study, we investigated BKV DNA in serum and plasma and urine specimens by real-time polym...
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| Published in: | Transplantation proceedings Vol. 50; no. 5; pp. 1510 - 1513 |
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| Main Authors: | , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
United States
Elsevier Inc
01-06-2018
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | BK virus (BKV) is a common human polyomavirus and causes latent infection. Especially in immunosuppressive patients, early diagnosis and treatment are very important in reducing the high mortality rate. In this study, we investigated BKV DNA in serum and plasma and urine specimens by real-time polymerase chain reaction (PCR) method in allogeneic hematopoietic stem cell transplantation patients.
BKV DNA was isolated in QIAsymphony SP/AS (Hilden, Germany) equipment using QIAsymphony DSP Virus/Pathogen Midi Kit, Version 1 (QIAGEN, Hilden, Germany) in urine and serum samples collected from 100 patients post-transplantation. Artus BKV QS-RGQ, V1 (Qiagen, Hilden, Germany) kit for BKV PCR was prepared according to the company recommendations and loaded on Qiagen Rotor Gene (Hilden, Germany). It was evaluated with PCR, and >27 copies/mL was considered as positive.
BKV DNA was positive in 57% of the urine specimens obtained; only 25% of the patients were found to have a significant BKV burden in the urine according to the American Society of Transplantation, suggesting a risk of developing nephropathy. Serum samples of the same patients were negative for BKV DNA in 94 cases and they were positive for BKV DNA at interval between 44 and 319 copies/mL in 6 patients, and none of the patients had clinically significant BKV DNA in serum samples.
Monitoring the viral load with urine samples were thought to be more convenient for the detection of BKV reactivation in our study.
•Real-time PCR method was used for the detection of BKV DNA and viral load monitoring in our study.•Our study found that monitoring the viral load with urine samples were thought to be more convenient for BKV reactivation detection in allo-HCST.•We observed in our study that hemorrhagic cystitis and severe hemorrhagic cystitis were not developed in any patient as a result of the levofloxacin use in prophylactic treatment in patients with clinically significant BK virus DNA level. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 0041-1345 1873-2623 |
| DOI: | 10.1016/j.transproceed.2017.12.056 |