An easy way to detect dengue virus using nanoparticle-antibody conjugates

The aim of the present research is to propose a new method based on localized surface plasmon resonance (LSPR) for fast dengue virus detection. A pool with four dengue serotypes (DENV-1, -2, -3, -4) was detected through antigen-antibody binding using gold nanoparticles (AuNPs) as signaling antibody...

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Bibliographic Details
Published in:	Virology (New York, N.Y.) Vol. 513; pp. 85 - 90
Main Authors:	Basso, Caroline R., Tozato, Claudia C., Crulhas, Bruno P., Castro, Gustavo R., Junior, João Pessoa A., Pedrosa, Valber A.
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01-01-2018
Subjects:	Antibodies, Viral - metabolism Biosensing Techniques - methods Biosensor Dengue - diagnosis Dengue Virus - isolation & purification Diagnostic Tests, Routine - methods Fast detection Infectious diagnostics Metal Nanoparticles Reproducibility of Results Sensitivity and Specificity Surface Plasmon Resonance Biosensor Infectious diagnostics Fast detection
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Summary:	The aim of the present research is to propose a new method based on localized surface plasmon resonance (LSPR) for fast dengue virus detection. A pool with four dengue serotypes (DENV-1, -2, -3, -4) was detected through antigen-antibody binding using gold nanoparticles (AuNPs) as signaling antibody carriers. Such result was confirmed through surface plasmon resonance (SPR), transmission electron microcopy (TEM), and dynamic light scattering (DLS) techniques. The limit of detection was calculated for TCID50 107 demonstrating a linear correlation between viral concentration and number of cells with an r2 value of > 0.993. The assay presented good sensibility and reproducibility of results and the negative controls were not mistakenly detected. This design requires no pretreatment or high trained person. In the future, it can be used in commercial antibody detection kits. •Nanoparticle capable of rapidly diagnosing dengue virus.•High specificity and stability.•Low cost when comparable with traditional analysis.
ISSN:	0042-6822 1096-0341
DOI:	10.1016/j.virol.2017.10.001