Genotoxic and mutagenic assessment of spinosad using bioassays with Tradescantia pallida and Drosophila melanogaster

Genotoxic and mutagenic assessment of spinosad using bioassays with Tradescantia pallida and Drosophila melanogaster

Spinosad (SPN) is a naturally-occurring insecticide obtained from the fermentation process of the actinomycete Saccharopolyspora spinosa. Owing to the larvicidal action, the compound has been used in the control of Aedes aegypti. As a new insecticide commercially available in the market, few data ar...

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Bibliographic Details
Published in:Chemosphere (Oxford) Vol. 222; pp. 503 - 510
Main Authors: Mendonça, Tarcísio Paiva, Davi de Aquino, Jéssica, Junio da Silva, Weverson, Mendes, Daniele Ruela, Campos, Carlos Fernando, Vieira, Jéssica Soares, Barbosa, Nathalya Pereira, Carvalho Naves, Maria Paula, Olegário de Campos Júnior, Edimar, Alves de Rezende, Alexandre Azenha, Spanó, Mário Antônio, Bonetti, Ana Maria, Vieira Santos, Vanessa Santana, Pereira, Boscolli Barbosa, Resende de Morais, Cássio
Format: Journal Article
Language:English
Published: England Elsevier Ltd 01-05-2019
Subjects:
Animals
Biolarvicides
Drosophila melanogaster - drug effects
Drosophila melanogaster - genetics
Drosophila Proteins
Drug Combinations
Female
Genotoxicity
Insecticides - toxicity
Larva - drug effects
Macrolides - toxicity
Male
Micronucleus Tests
Mutagenesis
Mutagenicity Tests - methods
Mutagens - toxicity
SMART
Spinosad
Trad-MCN
Tradescantia - drug effects
Tradescantia - genetics
Trad-MCN
Biolarvicides
Spinosad
Mutagenesis
SMART
Genotoxicity
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Summary:Spinosad (SPN) is a naturally-occurring insecticide obtained from the fermentation process of the actinomycete Saccharopolyspora spinosa. Owing to the larvicidal action, the compound has been used in the control of Aedes aegypti. As a new insecticide commercially available in the market, few data are reported on genotoxic effects in non-target organisms. The objective of the present study was to evaluate the mutagenic effect of SPN through the Micronucleus Test in Tradescantia pallida (Trad-MCN) and using the mutation and somatic recombination test in Drosophila melanogaster (SMART). At the Trad-MCN, after acclimatization (24 h), T. pallida stems were submitted to chronic treatment with SPN at concentrations of 0.156; 0.312; 0.625; 1.25 and 2.5 g/L solution for 24 h, followed by a recovery period. In SMART, considering the third stage larvae, offspring resulting from the ST and HB crossing were placed on chronic treatment (48 h) with 0.039; 0.078 and 0.156 μg/mL of SPN solution. No mutagenic effect was observed at any of the evaluated concentrations in SMART. Additionally, SPN is more toxic after metabolism via CYP6A2 (cytochrome P450) in D. melanogaster. However, SPN at the concentrations of 0.625; 1.25 and 2.5 g/L was able to induce high frequency of micronuclei in T. pallida. Under the experimental conditions of T. pallida in the present study, SPN caused genotoxic activity. [Display omitted] •Genotoxicity of spinosad was evaluated in Tradescantia pallida.•Mutagenicity of spinosad was evaluated in Drosophila melanogaster.•Spinosad is more toxic after metabolism via CYP6A2 (cytochrome P450).•Spinosad was not mutagenic in Drosophila melanogaster.•Spinosad was genotoxic in Tradescantia pallida.
ISSN:0045-6535
1879-1298
DOI:10.1016/j.chemosphere.2019.01.182