Bacterial target-specific photocatalyst for the enhancement of antibacterial property to targets

A bacterial target-specific TiO2 photocatalyst was developed for the selective inactivation of bacteria in an aqueous environment. The bacterial specific antibody was conjugated with TiO2 and this TiO2-antibody composite achieved an antibacterial effect which targets specific bacteria with short UV...

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Bibliographic Details
Published in:	Applied catalysis. B, Environmental Vol. 148-149; pp. 568 - 572
Main Authors:	Song, Min Young, Jung, Hyoun Duk, Jurng, Jongsoo, Kim, Byoung Chan
Format:	Journal Article
Language:	English
Published:	Kidlington Elsevier B.V 27-04-2014 Elsevier
Subjects:	Affinity Antibacterial effect Antibodies Antibody Antiinfectives and antibacterials Bacteria Catalysis Chemistry Composite Escherichia coli Exact sciences and technology General and physical chemistry Inactivation Particulate composites Photocatalysis Photocatalyst Photochemistry Physical chemistry of induced reactions (with radiations, particles and ultrasonics) Theory of reactions, general kinetics. Catalysis. Nomenclature, chemical documentation, computer chemistry TiO2 Titanium dioxide Composite Antibody Photocatalyst Antibacterial effect TiO2 Heterogeneous catalysis Transition element compounds TiO Antibacterial agent Titanium oxide Composite material Environmental protection
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Summary:	A bacterial target-specific TiO2 photocatalyst was developed for the selective inactivation of bacteria in an aqueous environment. The bacterial specific antibody was conjugated with TiO2 and this TiO2-antibody composite achieved an antibacterial effect which targets specific bacteria with short UV exposure time and low particle concentration compared to raw TiO2 particles. •TiO2 particles conjugated with bacterial target-specific antibody were prepared.•The TiO2-AbE can be attached on the surface of target bacterium.•The composite (TiO2-AbE) enhanced photokilling to only target bacterium.•The close contact of TiO2-AbE enhanced the photokilling of target bacteria. A bacterial target-specific titanium oxide (TiO2) photocatalyst was developed for the enhancement of selective inactivation of targeted bacteria. An antibacterial composition comprising TiO2 particles immobilized with a bacterial-specific antibody having affinity to bacteria of interest was prepared via a carbodiimide hydrochloride/N-hydroxysulfosuccinimide (EDC/NHS) coupling reaction between polyacrylic acid (PAA) coated TiO2 and an antibody. As a model case, an antibody to Escherichia coli was conjugated with the PAA-coated TiO2 (TiO2-AbE). We evaluated the enhancement of the antibacterial effect of TiO2-AbE against target E. coli, compared with its effect on other bacteria that lack affinity for the antibody used. The TiO2-AbE inactivated approximately 90% of the E. coli at 15min, whereas the raw TiO2 inactivated approximately 20% of the E. coli after the same period of time under UV irradiation. The TiO2-AbE did not show an enhanced antibacterial effect against non-target bacteria. We infer that close contact between TiO2 particles and E. coli, as a result of the specificity of the antibody, can enhance the direct transfer of reactive oxygen species (ROS) generated by TiO2 particles to the cellular surface under UV irradiation and result in rapid and efficient inactivation of the targeted bacteria. The strategy presented here will facilitate the combination of other receptors and TiO2 particles for the preparation of highly selective and photocatalytic composites to prevent or remediate contamination by unwanted bacteria in a wide variety of natural and man-made systems.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0926-3373 1873-3883 0926-3373
DOI:	10.1016/j.apcatb.2013.11.038