Allergic inflammation induced by a Penicillium chrysogenum conidia‐associated allergen extract in a murine model

Background: Recent evidence has shown that viable conidia from the fungus Penicillium chrysogenum induce allergic effects in mice. The present study was conducted to determine the specific allergic dose response of C57BL/6 mice to the protease extract, Pen ch, isolated from viable P. chrysogenum co...

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Published in:	Allergy (Copenhagen) Vol. 59; no. 7; pp. 758 - 765
Main Authors:	Schwab, C. J., Cooley, J. D., Jumper, C. J., Graham, S. C., Straus, D. C.
Format:	Journal Article
Language:	English
Published:	Oxford, UK Munksgaard International Publishers 01-07-2004 Blackwell
Subjects:	Administration, Intranasal allergens Allergens - administration & dosage Allergens - isolation & purification Allergic diseases allergic inflammation Animals Antigens, Fungal - administration & dosage Antigens, Fungal - isolation & purification Biological and medical sciences Bronchoalveolar Lavage Fluid - immunology Disease Models, Animal Dose-Response Relationship, Drug Electrophoresis, Polyacrylamide Gel - methods Enzyme-Linked Immunosorbent Assay - methods eosinophils Eosinophils - immunology Female Fundamental and applied biological sciences. Psychology Fundamental immunology Hypersensitivity - immunology immunoglobulin E Immunoglobulin E - blood Immunoglobulin E - immunology Immunoglobulin G - blood Immunoglobulin G - immunology Immunopathology Inflammation - immunology Injections, Intraperitoneal - methods Medical sciences Mice Mice, Inbred C57BL mold murine model Neutrophils - immunology Penicillium chrysogenum Penicillium chrysogenum - immunology sick building syndrome allergens; allergic inflammation; eosinophils; immunoglobulin E; mold; murine model Allergy Immunopathology Immunoglobulins Rodentia Granulocyte Sick building syndrome Extract Inflammation Penicillium chrysogenum; sick building syndrome Eosinophil Penicillium chrysogenum Fungi Vertebrata Mammalia Immunology Mouse Animal Models Fungi Imperfecti Thallophyta Allergen Conidia
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Summary:	Background: Recent evidence has shown that viable conidia from the fungus Penicillium chrysogenum induce allergic effects in mice. The present study was conducted to determine the specific allergic dose response of C57BL/6 mice to the protease extract, Pen ch, isolated from viable P. chrysogenum conidia. Methods: Mice were treated with primary intraperitoneal (IP) injections of 10 or 100 μg of Pen ch adsorbed to alum, followed by weekly IP injections of 0.1, 1.0, or 10.0 μg Pen ch with alum for 4 weeks, and with 10.0 μg of Pen ch by intranasal (IN) inoculations the final 2 weeks before killing. Results: Intraperitoneal injections of 10 and 100 μg of Pen ch for 5 weeks followed by 2 weeks of IN instillation of 10 μg induced significant increases of total serum immunoglobulin (Ig)E and IgG1. Bronchoalveolar lavage cell counts revealed increased numbers of eosinophils and neutrophils. Histopathological examination of lungs detected perivascular inflammation by eosinophils and neutrophils and increased mucous production. Conclusions: The data presented in this study indicate that sensitization to protease allergens released by viable P. chrysogenum conidia in vivo induce a strong allergic inflammatory response in a murine model, which could have implications for people exposed to high levels of conidia of this organism.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0105-4538 1398-9995
DOI:	10.1111/j.1398-9995.2004.00481.x