Virulence genes in Escherichia coli isolates from commercialized saltwater mussels Mytella guyanensis (Lamarck, 1819)

The isolation of Escherichia coli from food is a major concern. Pathogenic strains of these bacteria cause diseases which range from diarrhea to hemolytic-uremic syndrome. Therefore the virulence genes in E. coli isolates from the mussel ( Mytella guyanensis) commercialized in Cachoeira, Bahia, Braz...

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Published in:	Brazilian journal of biology Vol. 79; no. 4; pp. 625 - 628
Main Authors:	Barbosa, C A, Conceição, T A, Baliza, M D, Camilo, V M A, Juiz, P J L, Silva, I M M
Format:	Journal Article
Language:	English
Published:	Brazil Association of the Brazilian Journal of Biology 01-11-2019 Instituto Internacional de Ecologia
Subjects:	Bacteria BIOLOGY Brain coliforme termotolerante Coliforms Commercialization Conditioning Consumption Cooking Developing countries Diarrhea E coli Escherichia coli Food contamination & poisoning Food processing Gene elt Genes Glycerol Infections Laboratories LDCs Methylene blue Microbiological analysis Microbiology Mollusks molusco bivalve Mussels Mytella guyanensis Pathogens PCR Polymerase chain reaction Saline water Strains (organisms) Supermarkets Toxins Virulence Brasil Brazil molusco bivalve elt gene coliforme termotolerante thermotolerant coliform PCR Gene elt bivalve mollusk
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Summary:	The isolation of Escherichia coli from food is a major concern. Pathogenic strains of these bacteria cause diseases which range from diarrhea to hemolytic-uremic syndrome. Therefore the virulence genes in E. coli isolates from the mussel ( Mytella guyanensis) commercialized in Cachoeira, Bahia, Brazil were investigated. Samples were purchased from four vendors: two from supermarkets and two from fair outlets. They were conditioned into isothermal boxes with reusable ice and transported to the laboratory for analysis. E. coli strains were isolated in eosin methylene blue agar, preserved in brain-heart infusion medium with 15% glycerol and stored at -20 °C, after microbiological analysis. Virulence genes in the isolated strains were identified by specific primers, with Polymerase Chain Reaction. Twenty-four isolates were obtained, with a prevalence of elt gene, typical from enterotoxigenic infection, in 75% of the isolates. The stx and bfpA genes, prevalent in enterohemorragic and enteropathogenic E. coli, respectively, were not detected. The occurrence of elt virulence-related gene in the E. coli isolates of Mytella guyanensis reveals urgent improvement in food processing, including good handling practices, adequate storage and cooking before consumption, to ensure consumer's health.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1519-6984 1678-4375 1678-4375
DOI:	10.1590/1519-6984.185930