In vivo expression of rat insulin after intravenous administration of the liposome-entrapped gene for rat insulin I

A recombinant plasmid encoding rat preproinsulin I was encapsulated in large liposomes and intravenously injected in rats. Glycemia and blood, splenic, and hepatic insulin were assayed at various times beginning 6 hr after inoculation. The results were compared with controls that had received (i) em...

Full description

Saved in:

Bibliographic Details
Published in:	Proceedings of the National Academy of Sciences - PNAS Vol. 80; no. 4; pp. 1068 - 1072
Main Authors:	Nicolau, C, Le Pape, A, Soriano, P, Fargette, F, Juhel, M.F
Format:	Journal Article
Language:	English
Published:	United States National Academy of Sciences of the United States of America 01-02-1983 National Acad Sciences
Subjects:	animal breeding animal genetics Animals Blood Blood glucose Blood Glucose - metabolism diet-related diseases DNA DNA, Recombinant Gene Expression Regulation human nutrition Insulin Insulin - genetics Kupffer cells Liposomes Liposomes - administration & dosage Liver Liver - physiology Plasmids Radioactive decay Rats Spleen Spleen - physiology Time Factors Tissue Distribution Transformation, Genetic
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	A recombinant plasmid encoding rat preproinsulin I was encapsulated in large liposomes and intravenously injected in rats. Glycemia and blood, splenic, and hepatic insulin were assayed at various times beginning 6 hr after inoculation. The results were compared with controls that had received (i) empty liposomes, (ii) liposomes carrying the Escherichia coli pBR322 plasmid, (iii) the free rat insulin I gene, and (iv) no injection at all. Whereas all controls showed unchanged glucose and insulin levels, the treated animals had, 6 hr after inoculation, a blood glucose level of 72± 5 mg of glucose/100 ml of blood as compared with 107± 2 mg/ml for controls. Radioimmunoassay of blood insulin gave 61± 8 microunits/ml as compared with 43± 5 microunits/ml for controls and the spleen and liver values were 242± 22 and 204± 20 microunits/g of tissue, respectively, as compared with 112± 20 and 87± 15 microunits/g in controls. External γ -camera imaging of the organ uptake of 111In-labeled liposomes permitted study of the kinetics and extent of uptake of the liposomes by spleen and liver, results that support the findings concerning insulin synthesis in the two organs.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.80.4.1068