Search Results - "Journal of pharmacological and toxicological methods"

Seizure liability assessments using the hippocampal tissue slice: Comparison of non-clinical species by Accardi, Michael V., Huang, Hai, Authier, Simon

“…Traditionally, rat hippocampal tissue slice models are used as an in vitro electrophysiology assay to assess seizurogenic potential in early drug development…”
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Establishment of a dipeptidyl peptidases (DPP) 8/9 expressing cell model for evaluating the selectivity of DPP4 inhibitors by Huan, Yi, Jiang, Qian, Liu, Jing-long, Shen, Zhu-fang

“…Dipeptidyl peptidases (DPPs) 8 and 9 are homologous, cytoplasmic postproline-cutting enzymes, which have similar enzymatic activity and preferred substrates as…”
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Post natal cardiovascular assessments in nonclinical pediatric studies using the jacketed external telemetry in beagles for juvenile toxicology studies by Matheson, Rommel, Adamo, Maria, Robinson, Keith, Norton, Kevin

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Current methodologies used for evaluation of intestinal permeability and absorption by Balimane, Praveen V, Chong, Saeho, Morrison, Richard A

“…This review article will focus on the various techniques that are currently employed by drug discovery scientists in evaluating permeability/absorption of drug…”
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The Comprehensive in Vitro Proarrhythmia Assay (CiPA) initiative — Update on progress by Colatsky, Thomas, Fermini, Bernard, Gintant, Gary, Pierson, Jennifer B., Sager, Philip, Sekino, Yuko, Strauss, David G., Stockbridge, Norman

“…The implementation of the ICH S7B and E14 guidelines has been successful in preventing the introduction of potentially torsadogenic drugs to the market, but it…”
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An evaluation of 30 clinical drugs against the comprehensive in vitro proarrhythmia assay (CiPA) proposed ion channel panel by Crumb, William J., Vicente, Jose, Johannesen, Lars, Strauss, David G.

“…The Comprehensive in vitro Proarrhythmia Assay (CiPA) is intended to address the misidentification of drug-associated torsade de pointes risk based solely on…”
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An inexpensive system for evaluating the tussive and anti-tussive properties of chemicals in conscious, unrestrained guinea pigs by Daller, J.R., Wong, J., Brooks, B.D., McKee, J.S.

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A new paradigm for drug-induced torsadogenic risk assessment using human iPS cell-derived cardiomyocytes by Ando, Hiroyuki, Yoshinaga, Takashi, Yamamoto, Wataru, Asakura, Keiichi, Uda, Takaaki, Taniguchi, Tomohiko, Ojima, Atsuko, Shinkyo, Raku, Kikuchi, Kiyomi, Osada, Tomoharu, Hayashi, Seiji, Kasai, Chieko, Miyamoto, Norimasa, Tashibu, Hiroyuki, Yamazaki, Daiju, Sugiyama, Atsushi, Kanda, Yasunari, Sawada, Kohei, Sekino, Yuko

“…Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are anticipated to be a useful tool for conducting proarrhythmia risk assessments of…”
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Experimental diabetes induced by alloxan and streptozotocin: The current state of the art by Radenković, Miroslav, Stojanović, Marko, Prostran, Milica

“…Diabetes mellitus is a chronic metabolic disorder with a high prevalence worldwide. Animal models of diabetes represent an important tool in diabetes…”
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Cardiotoxicity screening with simultaneous optogenetic pacing, voltage imaging and calcium imaging by Dempsey, Graham T., Chaudhary, Khuram W., Atwater, Nicholas, Nguyen, Cuong, Brown, Barry S., McNeish, John D., Cohen, Adam E., Kralj, Joel M.

“…The Comprehensive in vitro Proarrhythmia Assay (CiPA) initiative seeks an in vitro test to accurately predict clinical Torsades de Pointes (TdP). We developed…”
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A murine model of type 2 diabetes mellitus developed using a combination of high fat diet and multiple low doses of streptozotocin treatment mimics the metabolic characteristics of type 2 diabetes mellitus in humans by Nath, Sayantan, Ghosh, Sankar Kumar, Choudhury, Yashmin

“…A murine model of type 2 diabetes mellitus was used to compare the antidiabetic effects of the dipeptidyl peptidase-4 (DPP4) inhibitor vildagliptin and…”
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Use of the viability reagent PrestoBlue in comparison with alamarBlue and MTT to assess the viability of human corneal epithelial cells by Xu, Manlong, McCanna, David J., Sivak, Jacob G.

“…PrestoBlue is a new resazurin based reagent to assess cell viability and cytotoxicity. It is claimed to be a fast and highly sensitive assay. Here, we compared…”
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Improvement of acquisition and analysis methods in multi-electrode array experiments with iPS cell-derived cardiomyocytes by Asakura, Keiichi, Hayashi, Seiji, Ojima, Atsuko, Taniguchi, Tomohiko, Miyamoto, Norimasa, Nakamori, Chiaki, Nagasawa, Chiho, Kitamura, Tetsuo, Osada, Tomoharu, Honda, Yayoi, Kasai, Chieko, Ando, Hiroyuki, Kanda, Yasunari, Sekino, Yuko, Sawada, Kohei

“…Multi-electrode array (MEA) systems and human induced pluripotent stem (iPS) cell-derived cardiomyocytes are frequently used to characterize the…”
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Computational tools for fitting the Hill equation to dose–response curves by Gadagkar, Sudhindra R., Call, Gerald B.

“…Many biological response curves commonly assume a sigmoidal shape that can be approximated well by means of the 4-parameter nonlinear logistic equation, also…”
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Real time monitoring and quantification of reactive oxygen species in breast cancer cell line MCF-7 by 2′,7′–dichlorofluorescin diacetate (DCFDA) assay by Figueroa, Daniela, Asaduzzaman, Mohammad, Young, Fiona

“…The detection of reactive oxygen species (ROS) using 2′,7′–dichlorofluorescin diacetate (DCFDA) is commonly performed by a single measurement of fluorescence…”
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Comparison of PrestoBlue and MTT assays of cellular viability in the assessment of anti-proliferative effects of plant extracts on human endothelial cells by Boncler, Magdalena, Różalski, Marek, Krajewska, Urszula, Podsędek, Anna, Watala, Cezary

“…PrestoBlue (PB) is a new, simple and extremely fast live assay to monitor cell viability and cytotoxicity. Herein, we compared two in vitro cytotoxicity…”
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The utility of the minipig as an animal model in regulatory toxicology by Bode, Gerd, Clausing, Peter, Gervais, Frederic, Loegsted, Jeanet, Luft, Jörg, Nogues, Vicente, Sims, Jennifer

“…In this article we review the value and utility of the minipig as an animal model in regulatory toxicity testing. Our review is based on detailed consideration…”
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Marble-burying behavior test as a murine model of compulsive-like behavior by Dixit, Pankaj Vinod, Sahu, Rohit, Mishra, Dinesh Kumar

“…Object burying by rodents is a popular screening tool for anxiolytic agents. However, modulation of marble-burying by serotonin reuptake inhibitors prompted…”
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Best practice considerations for nonclinical in vivo cardiovascular telemetry studies in non-rodent species: Delivering high quality QTc data to support ICH E14/S7B Q&As by Rossman, Eric I., Wisialowski, Todd A., Vargas, Hugo M., Valentin, Jean-Pierre, Rolf, Michael G., Roche, Brian M., Riley, Steve, Pugsley, Michael K., Nichols, Jill, Li, Dingzhou, Leishman, Derek J., Kleiman, Robert B., Greiter-Wilke, Andrea, Gintant, Gary A., Engwall, Michael J., Delaunois, Annie, Authier, Simon

“…The ICH E14/S7B Questions and Answers (Q&As) guideline introduces the concept of a “double negative” nonclinical scenario (negative hERG assay and negative in…”
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Safety screening in early drug discovery: An optimized assay panel by Bendels, Stefanie, Bissantz, Caterina, Fasching, Bernhard, Gerebtzoff, Grégori, Guba, Wolfgang, Kansy, Manfred, Migeon, Jacques, Mohr, Susanne, Peters, Jens-Uwe, Tillier, Fabien, Wyler, René, Lerner, Christian, Kramer, Christian, Richter, Hans, Roberts, Sonia

“…Several factors contribute to the development failure of novel pharmaceuticals, one of the most important being adverse effects in pre-clinical and clinical…”
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