Search Results - "Journal of immunological methods"

Aging and the immune system: An overview by Sadighi Akha, Amir A.

“…The world is witnessing a rapid demographic shift towards an older population, a trend with major medical, social, economic and political implications. Aging…”
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A high-throughput, bead-based, antigen-specific assay to assess the ability of antibodies to induce complement activation by Fischinger, Stephanie, Fallon, Jonathan K., Michell, Ashlin R., Broge, Thomas, Suscovich, Todd J., Streeck, Hendrik, Alter, Galit

“…The complement system plays a critical role in innate immune defense against pathogens, both via non-specific direct pathogen recognition and killing or via…”
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A versatile high-throughput assay to characterize antibody-mediated neutrophil phagocytosis by Karsten, Christina B., Mehta, Nickita, Shin, Sally A., Diefenbach, Thomas J., Slein, Matthew D., Karpinski, Wiktor, Irvine, Edward B., Broge, Thomas, Suscovich, Todd J., Alter, Galit

“…Neutrophils, the most abundant white blood cell, play a critical role in anti-pathogen immunity via phagocytic clearance, secretion of enzymes and…”
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Optimization and validation of the TZM-bl assay for standardized assessments of neutralizing antibodies against HIV-1 by Sarzotti-Kelsoe, Marcella, Bailer, Robert T., Turk, Ellen, Lin, Chen-li, Bilska, Miroslawa, Greene, Kelli M., Gao, Hongmei, Todd, Christopher A., Ozaki, Daniel A., Seaman, Michael S., Mascola, John R., Montefiori, David C.

“…The TZM-bl assay measures antibody-mediated neutralization of HIV-1 as a function of reductions in HIV-1 Tat-regulated firefly luciferase (Luc) reporter gene…”
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Isolation of biologically-active exosomes from human plasma by Muller, Laurent, Hong, Chang-Sook, Stolz, Donna B., Watkins, Simon C., Whiteside, Theresa L.

“…Effects of exosomes present in human plasma on immune cells have not been examined in detail. Immunological studies with plasma-derived exosomes require their…”
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The choice of phorbol 12-myristate 13-acetate differentiation protocol influences the response of THP-1 macrophages to a pro-inflammatory stimulus by Lund, Maria E., To, Joyce, O'Brien, Bronwyn A., Donnelly, Sheila

“…The human monocytic cell line, THP-1, is the most widely used model for primary human monocytes/macrophages. This is because, following differentiation using…”
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Comparative analysis of the efficiency and specificity of myeloid-Cre deleting strains using ROSA-EYFP reporter mice by Abram, Clare L., Roberge, Gray L., Hu, Yongmei, Lowell, Clifford A.

“…Since the first example of conditional gene targeting in mice in 1994, the use of Cre recombinase and loxP flanked sequences has become an invaluable technique…”
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Standardized protocols for differentiation of THP-1 cells to macrophages with distinct M(IFNγ+LPS), M(IL-4) and M(IL-10) phenotypes by Baxter, E.W., Graham, A.E., Re, N.A., Carr, I.M., Robinson, J.I., Mackie, S.L., Morgan, A.W.

“…In vitro models of differing macrophage functions are useful since human monocyte–derived macrophages are short–lived, finite and vary from donor to donor…”
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A new background subtraction method for Western blot densitometry band quantification through image analysis software by Gallo-Oller, Gabriel, Ordoñez, Raquel, Dotor, Javier

“…Since its first description, Western blot has been widely used in molecular labs. It constitutes a multistep method that allows the detection and/or…”
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Optimization of the ligature-induced periodontitis model in mice by Abe, Toshiharu, Hajishengallis, George

“…Periodontitis is a prevalent oral inflammatory disease that leads to alveolar bone loss and may exert an adverse impact on systemic health. Experimental animal…”
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Immunoinformatics-guided designing of epitope-based subunit vaccine from Pilus assembly protein of Acinetobacter baumannii bacteria by Mahapatra, Soumya Ranjan, Dey, Jyotirmayee, Jaiswal, Aryan, Roy, Riya, Misra, Namrata, Suar, Mrutyunjay

“…Acinetobacter baumannii, a prominent pathogen responsible for chronic infections in the blood, urinary tract, and lungs, has a high mortality due to its…”
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SARS-CoV-2-specific ELISA development by Roy, Vicky, Fischinger, Stephanie, Atyeo, Caroline, Slein, Matthew, Loos, Carolin, Balazs, Alejandro, Luedemann, Corinne, Astudillo, Michael Gerino, Yang, Diane, Wesemann, Duane R., Charles, Richelle, Lafrate, A. John, Feldman, Jared, Hauser, Blake, Caradonna, Tim, Miller, Tyler E., Murali, Mandakolathur R., Baden, Lindsey, Nilles, Eric, Ryan, Edward, Lauffenburger, Douglas, Beltran, Wilfredo Garcia, Alter, Galit

“…Critical to managing the spread of COVID-19 is the ability to diagnose infection and define the acquired immune response across the population. While genomic…”
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Comparative analyses of SARS-CoV-2 binding (IgG, IgM, IgA) and neutralizing antibodies from human serum samples by Mazzini, Livia, Martinuzzi, Donata, Hyseni, Inesa, Benincasa, Linda, Molesti, Eleonora, Casa, Elisa, Lapini, Giulia, Piu, Pietro, Trombetta, Claudia Maria, Marchi, Serena, Razzano, Ilaria, Manenti, Alessandro, Montomoli, Emanuele

“…A newly identified coronavirus, named SARS-CoV-2, emerged in December 2019 in Hubei Province, China, and quickly spread throughout the world; so far, it has…”
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Methods to determine intestinal permeability and bacterial translocation during liver disease by Wang, Lirui, Llorente, Cristina, Hartmann, Phillipp, Yang, An-Ming, Chen, Peng, Schnabl, Bernd

“…Liver disease is often times associated with increased intestinal permeability. A disruption of the gut barrier allows microbial products and viable bacteria…”
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Multiplexed Fc array for evaluation of antigen-specific antibody effector profiles by Brown, Eric P., Dowell, Karen G., Boesch, Austin W., Normandin, Erica, Mahan, Alison E., Chu, Thach, Barouch, Dan H., Bailey-Kellogg, Chris, Alter, Galit, Ackerman, Margaret E.

“…Antibodies are widely considered to be a frequent primary and often mechanistic correlate of protection of approved vaccines; thus evaluating the antibody…”
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ELDA: Extreme limiting dilution analysis for comparing depleted and enriched populations in stem cell and other assays by Hu, Yifang, Smyth, Gordon K.

“…ELDA is a software application for limiting dilution analysis (LDA), with particular attention to the needs of stem cell assays. It is the first limiting…”
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Methodological comparison of FACS and MACS isolation of enriched microglia and astrocytes from mouse brain by Pan, Jie, Wan, Jun

“…Microglia and astrocytes, the two innate cells in CNS, are thought to protect and remodel of synapses for proper maintenance and plasticity of neuronal…”
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Quantification of apoptosis and necroptosis at the single cell level by a combination of Imaging Flow Cytometry with classical Annexin V/propidium iodide staining by Pietkiewicz, Sabine, Schmidt, Jörn H., Lavrik, Inna N.

“…Precisely identifying the type of programmed cell death is one of the key questions in contemporary biomedical research. We developed a straightforward…”
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Measuring IL-6 and sIL-6R in serum from patients treated with tocilizumab and/or siltuximab following CAR T cell therapy by Chen, Fang, Teachey, David T., Pequignot, Edward, Frey, Noelle, Porter, David, Maude, Shannon L., Grupp, Stephan A., June, Carl H., Melenhorst, Jan J., Lacey, Simon F.

“…T cells expressing a CD19-specific chimeric antigen receptor (CAR19) are demonstrating remarkable efficacy in hematologic malignancies. Treatment is often…”
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Impact of blood storage and sample handling on quality of high dimensional flow cytometric data in multicenter clinical research by Diks, A.M., Bonroy, C., Teodosio, C., Groenland, R.J., de Mooij, B., de Maertelaere, E., Neirynck, J., Philippé, J., Orfao, A., van Dongen, J.J.M., Berkowska, M.A.

“…Obtaining reliable and reproducible high quality data in multicenter clinical research settings requires design of optimal standard operating procedures. While…”
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