Arabidopsis SGS2 and SGS3 Genes Are Required for Posttranscriptional Gene Silencing and Natural Virus Resistance

Posttranscriptional gene silencing (PTGS) in plants results from the degradation of mRNAs and shows phenomenological similarities with quelling in fungi and RNAi in animals. Here, we report the isolation of sgs2 and sgs3 Arabidopsis mutants impaired in PTGS. We establish a mechanistic link between P...

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Bibliographic Details
Published in:	Cell Vol. 101; no. 5; pp. 533 - 542
Main Authors:	Mourrain, Philippe, Béclin, Christophe, Elmayan, Taline, Feuerbach, Frank, Godon, Christian, Morel, Jean-Benoit, Jouette, David, Lacombe, Anne-Marie, Nikic, Snezana, Picault, Nathalie, Rémoué, Karine, Sanial, Mathieu, Vo, Truy-Anh, Vaucheret, Hervé
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 26-05-2000 Elsevier
Subjects:	Amino Acid Sequence Arabidopsis Arabidopsis - enzymology Arabidopsis - genetics Arabidopsis Proteins Arabidopsis thaliana Base Sequence Cellular Biology Chromosome Mapping Cloning, Molecular Cucumovirus DNA, Plant EGO-1 protein Gene Silencing Genes, Plant Life Sciences Lycopersicon esculentum - enzymology Molecular Sequence Data Mutagenesis Plant Diseases - virology Plant Proteins - genetics Plant Proteins - metabolism Potyvirus QDE-1 protein RNA Processing, Post-Transcriptional RNA Replicase - genetics RNA Replicase - metabolism SGS2 gene SGS2 protein SGS3 gene Tobamovirus GENETIQUE
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Summary:	Posttranscriptional gene silencing (PTGS) in plants results from the degradation of mRNAs and shows phenomenological similarities with quelling in fungi and RNAi in animals. Here, we report the isolation of sgs2 and sgs3 Arabidopsis mutants impaired in PTGS. We establish a mechanistic link between PTGS, quelling, and RNAi since the Arabidopsis SGS2 protein is similar to an RNA-dependent RNA polymerase like N. crassa QDE-1, controlling quelling, and C. elegans EGO-1, controlling RNAi. In contrast, SGS3 shows no significant similarity with any known or putative protein, thus defining a specific step of PTGS in plants. Both sgs2 and sgs3 mutants show enhanced susceptibility to virus, definitively proving that PTGS is an antiviral defense mechanism that can also target transgene RNA for degradation.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0092-8674 1097-4172
DOI:	10.1016/S0092-8674(00)80863-6