Identification of Myopia-Related Marker Proteins in Tilapia Retinal, RPE, and Choroidal Tissue following Induced Form Deprivation

Purpose: Experimentally induced myopia is characterized by axial elongation of the eye. The molecular pathways leading to this condition are largely unknown, even though many candidate proteins have been proposed to be involved in this process. This study has identified proteins that were differenti...

Full description

Saved in:

Bibliographic Details
Published in:	Current eye research Vol. 34; no. 11; pp. 966 - 975
Main Authors:	Jostrup, Rasmus, Shen, Wei, Burrows, Jeffrey T. A., Sivak, Jacob G., McConkey, Brendan J., Singer, Thomas D.
Format:	Journal Article
Language:	English
Published:	England Informa UK Ltd 01-11-2009 Taylor & Francis
Subjects:	Amino Acid Sequence Animal model Animals Annexin A5 - genetics Annexin A5 - metabolism Base Sequence Biomarkers - metabolism Chaperonin Containing TCP-1 - metabolism Choroid - metabolism Cichlids Cloning, Molecular Disease Models, Animal Down-Regulation Electrophoresis, Gel, Two-Dimensional Eye Proteins - genetics Eye Proteins - metabolism Fish Proteins - genetics Fish Proteins - metabolism Form deprivation Form Perception Gelsolin - metabolism Molecular Sequence Data Myopia Myopia - genetics Myopia - metabolism Proteomics Retina - metabolism Retinal Pigment Epithelium - metabolism Sensory Deprivation Sequence Homology, Amino Acid Tandem Mass Spectrometry Tilapia
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Purpose: Experimentally induced myopia is characterized by axial elongation of the eye. The molecular pathways leading to this condition are largely unknown, even though many candidate proteins have been proposed to be involved in this process. This study has identified proteins that were differentially expressed in myopic and control combined retina, retinal pigment epithelium (RPE), and choroidal tissue in tilapia (Oreochromis niloticus). Methods: Form deprivation was used to induce myopia in tilapia (n = 3). In this initial study on tilapia retina, RPE and choroid, 2-D differential in gel electrophoresis (DIGE) and mass spectrometry were used to identify differentially expressed proteins. Homology-based gene cloning was used to obtain full sequence data for one of the identified proteins. Results: A total of 18 protein spots separated by 2-D electrophoresis exhibited statistically significant differences in expression between the myopic and contralateral control combined retinal, RPE, and choroidal tissue. Three proteins were identified at a significance level of p < 0.05, as annexin A5 (down-regulated 47%), Gelsolin (down-regulated 27%), and TCP-1 (CCT) (down-regulated 54%). DNA sequencing of tilapia annexin A5 shows an amino acid sequence identity of 84.5% with the homologous Japanese ricefish annexin max2. Conclusions: A proteomics approach has been used to identify differentially expressed proteins in form-deprived combined retinal, RPE, and choroidal tissue from myopic versus normal eyes. The identified proteins may be components of pathways involved in myopia pathogenesis.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0271-3683 1460-2202
DOI:	10.3109/02713680903244138