Platelets, endothelial cells and leukocytes contribute to the exercise-triggered release of extracellular vesicles into the circulation

Platelets, endothelial cells and leukocytes contribute to the exercise-triggered release of extracellular vesicles into the circulation

Physical activity initiates a wide range of multi-systemic adaptations that promote mental and physical health. Recent work demonstrated that exercise triggers the release of extracellular vesicles (EVs) into the circulation, possibly contributing to exercise-associated adaptive systemic signalling....

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Bibliographic Details
Published in:Journal of extracellular vesicles Vol. 8; no. 1; pp. 1615820 - n/a
Main Authors: Brahmer, Alexandra, Neuberger, Elmo, Esch-Heisser, Leona, Haller, Nils, Jorgensen, Malene Moeller, Baek, Rikke, Möbius, Wiebke, Simon, Perikles, Krämer-Albers, Eva-Maria
Format: Journal Article
Language:English
Published: Sweden Taylor & Francis 01-12-2019
John Wiley & Sons, Inc
Wiley
Subjects:
Adaptation
Anaerobic threshold
Antigen-presenting cells
Blood
Blood platelets
CD105 antigen
CD14 antigen
CD4 antigen
CD63 antigen
CD8 antigen
CD81 antigen
CD9 antigen
Cell surface
Circulatory system
Endothelial cells
Exercise
exosomes
Extracellular vesicles
Homeostasis
immunobead isolation
Leukocytes
Lymphocytes
Major histocompatibility complex
Metabolism
Monocytes
multiplex phenotyping
Phenotyping
Physical activity
Plasma
Platelets
size exclusion chromatography
Surface markers
United Kingdom > UK
United States > US
Germany
size exclusion chromatography
exosomes
multiplex phenotyping
Extracellular vesicles
exercise
plasma
immunobead isolation
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Summary:Physical activity initiates a wide range of multi-systemic adaptations that promote mental and physical health. Recent work demonstrated that exercise triggers the release of extracellular vesicles (EVs) into the circulation, possibly contributing to exercise-associated adaptive systemic signalling. Circulating EVs comprise a heterogeneous collection of different EV-subclasses released from various cell types. So far, a comprehensive picture of the parental and target cell types, EV-subpopulation diversity and functional properties of EVs released during exercise (ExerVs) is lacking. Here, we performed a detailed EV-phenotyping analysis to explore the cellular origin and potential subtypes of ExerVs. Healthy male athletes were subjected to an incremental cycling test until exhaustion and blood was drawn before, during, and immediately after the test. Analysis of total blood plasma by EV Array suggested endothelial and leukocyte characteristics of ExerVs. We further purified ExerVs from plasma by size exclusion chromatography as well as CD9-, CD63- or CD81-immunobead isolation to examine ExerV-subclass dynamics. EV-marker analysis demonstrated increasing EV-levels during cycling exercise, with highest levels at peak exercise in all EV-subclasses analysed. Phenotyping of ExerVs using a multiplexed flow-cytometry platform revealed a pattern of cell surface markers associated with ExerVs and identified lymphocytes (CD4, CD8), monocytes (CD14), platelets (CD41, CD42, CD62P), endothelial cells (CD105, CD146) and antigen presenting cells (MHC-II) as ExerV-parental cells. We conclude that multiple cell types associated with the circulatory system contribute to a pool of heterogeneous ExerVs, which may be involved in exercise-related signalling mechanisms and tissue crosstalk.
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ISSN:2001-3078
2001-3078
DOI:10.1080/20013078.2019.1615820