Biological interactions and attenuation of MPTP-induced toxicity in Drosophila melanogaster by Trans-astaxanthin

Biological interactions and attenuation of MPTP-induced toxicity in Drosophila melanogaster by Trans-astaxanthin

Trans-astaxanthin (TA) is a carotenoid with amphipathic chemical structure found in yeast, and aquatic organisms. It is known to possess both antioxidative and anti-inflammatory properties. This study was carried out to investigate the ameliorative action of TA on 1-methyl-4-phenyl-1,2,3,6-tetrahydr...

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Bibliographic Details
Published in:Neuroscience research Vol. 196; pp. 52 - 58
Main Authors: Omotayo, Tolulope, Otenaike, Titilayomi A., Adedara, Adeola Oluwatosin, Adeyemi, Oluwagbenga Eyitayo, Jonhnson, Titilayo O., Abolaji, Amos Olalekan
Format: Journal Article
Language:English
Published: Ireland Elsevier B.V 01-11-2023
Subjects:
Molecular docking
Oxidative stress
Parkinsonism
Trans-astaxanthin
Parkinsonism
Oxidative stress
Trans-astaxanthin
Molecular docking
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Summary:Trans-astaxanthin (TA) is a carotenoid with amphipathic chemical structure found in yeast, and aquatic organisms. It is known to possess both antioxidative and anti-inflammatory properties. This study was carried out to investigate the ameliorative action of TA on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced toxicity in Drosophila melanogaster (Fruit fly). The flies were orally treated with TA (2.5 mg/10 g diet) and/or MPTP (500 µM) for 5 days. Thereafter, we evaluated selected biomarkers of locomotor deficits (acetylcholinesterase (AChE) and negative geotaxis), oxidative stress (hydrogen peroxide (H2O2), protein carbonyls (PC)), antioxidants (total thiols (T-SH), non-protein thiols, glutathione-S-transferase (GST) and catalase), and inflammation (nitric oxide (nitrite/nitrate) in the flies. Furthermore, we investigated molecular docking analysis of TA against Kelch-like ECH-associated protein 1 (Keap1)) of Homo sapiens and D. melanogaster. The results indicated that TA increased MPTP-induced decreased activities of AChE, GST, and catalase, as well as levels of non-protein thiols and T-SH compared with MPTP-treated flies (p < 0.05). Furthermore, TA attenuated inflammation, and improved locomotor deficit in the flies. The molecular docking data showed that TA had docking scores for binding both the Human and Drosophila Keap1, nearly closer to or higher than the standard inhibitor. The attenuating effects of TA against MPTP-induced toxicity could arise from its antioxidative and anti-inflammatory properties as well as its chemical structure. [Display omitted] •MPTP was used to model Parkinson’s disease-like condition in Drosophila melanogaster.•We found that Trans-astaxanthin (TA) ameliorates MPTP-induced toxicity in D. melanogaster.•The TA has docking scores for binding human and Drosophila and Keap1.•Attenuating effects of TA could be due to its antioxidative and anti-inflammatory properties and chemical structure.
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ISSN:0168-0102
1872-8111
DOI:10.1016/j.neures.2023.06.005