Detection of influenza virus by electrochemical surface plasmon resonance under potential modulation

In this study we report the development of a novel viral pathogen immunosensor technology based on the electrochemical modulation of the optical signal from a surface plasmon wave interacting with a redox dye reporter. The device is formed by incorporating a sandwich immunoassay onto the surface of...

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Bibliographic Details
Published in:	Applied optics. Optical technology and biomedical optics Vol. 58; no. 11; p. 2839
Main Authors:	Qatamin, Aymen H, Ghithan, Jafar H, Moreno, Monica, Nunn, Betty M, Jones, Keenan B, Zamborini, Francis P, Keynton, Robert S, O'Toole, Martin G, Mendes, Sergio B
Format:	Journal Article
Language:	English
Published:	United States 10-04-2019
Subjects:	Antibodies, Monoclonal - immunology Antigens, Viral - analysis Biosensing Techniques - instrumentation Immunoassay - instrumentation Influenza A Virus, H5N1 Subtype - immunology Limit of Detection Methylene Blue - chemistry Surface Plasmon Resonance - instrumentation
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Summary:	In this study we report the development of a novel viral pathogen immunosensor technology based on the electrochemical modulation of the optical signal from a surface plasmon wave interacting with a redox dye reporter. The device is formed by incorporating a sandwich immunoassay onto the surface of a plasmonic device mounted in a micro-electrochemical flow cell, where it is functionalized with a monoclonal antibody aimed to a specific target pathogen antigen. Once the target antigen is bound to the surface, it promotes the capturing of a secondary polyclonal antibody that has been conjugated with a redox-active methylene blue dye. The methylene blue displays a reversible change in the complex refractive index throughout a reduction-oxidation transition, which generates an optical signal that can be electrochemically modulated and detected at high sensitivity. For proof-of-principle measurements, we have targeted the hemagglutinin protein from the H5N1 avian influenza A virus to demonstrate the capabilities of our device for detection and quantification of a critical influenza antigen. Our experimental results of the EC-SPR-based immunosensor under potential modulation showed a 300 pM limit of detection for the H5N1 antigen.
ISSN:	2155-3165
DOI:	10.1364/ao.58.002839