Search Results - "Jonathan E. Poulton"

Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35 by Ahn, Young Ock, Zheng, Meiying, Bevan, David R., Esen, Asim, Shiu, Shin-Han, Benson, Jonas, Peng, Hsiao-Ping, Miller, Joseph T., Cheng, Chi-Lien, Poulton, Jonathan E., Shih, Ming-Che

“…The gene structure, evolutionary patterns, and organ-level expression of the 17 members of Arabidopsis thaliana Glycoside Hydrolase Family 35…”
Get full text

Investigation of the Microheterogeneity and Aglycone Specificity-Conferring Residues of Black Cherry Prunasin Hydrolases by Jiming Zhou, Hartmann, Stefanie, Brianne K. Shepherd, Jonathan E. Poulton

“…In black cherry (Prunus serotina Ehrh.) seed homogenates, (R)-amygdalin is degraded to HCN, benzaldehyde, and glucose by the sequential action of amygdalin…”
Get full text

Cyanogenesis in plants by Poulton, J.E. (University of Iowa, Iowa City, IA)

“…Several thousand plant species, including many economically important food plants, synthesize cyanogenic glycosides and cyanolipids. Upon tissue disruption,…”
Get full text

Arabidopsis thaliana β-Glucosidases BGLU45 and BGLU46 hydrolyse monolignol glucosides by Escamilla-Treviño, Luis L., Chen, Wei, Card, Marcella L., Shih, Ming-Che, Cheng, Chi-Lien, Poulton, Jonathan E.

“…Biochemical and molecular evidence are provided to support the hypothesis that Arabidopsis thaliana β-glucosidases BGLU45 (At1g61810) and BGLU46 (At1g61820)…”
Get full text

Molecular Analysis of (R)-(+)-Mandelonitrile Lyase Microheterogeneity in Black Cherry by Hu, Z, Poulton, J E

“…The flavoprotein (R)-(+)-mandelonitrile lyase (MDL; EC 4.1.2.10), which plays a key role in cyanogenesis in rosaceous stone fruits, occurs in black cherry…”
Get full text

An extracellular exo-β-(1,3)-glucanase from Pichia pastoris: Purification, characterization, molecular cloning, and functional expression by Xu, Zhiwei, Shih, Ming-Che, Poulton, Jonathan E.

“…An extracellular exo-β-(1,3)-glucanase (designated EXG1) was purified to apparent homogeneity from Pichia pastoris X-33 cultures by ammonium sulfate…”
Get full text

Tissue and Subcellular Localization of Enzymes Catabolizing (R)-Amygdalin in Mature Prunus serotina Seeds by SWAIN, E, CHUN PING LI, POULTON, J. E

“…In black cherry (Prunus serotina Ehrh.) homogenates, (R)-amygdalin is catabolized to HCN, benzaldehyde, and D-glucose by the sequential action of amygdalin…”
Get full text

Development of the potential of cyanogenesis in maturing black cherry (Prunus serotina Ehrh.) fruits by Swain, E. (University of Iowa, Iowa City, IA), Li, C.P, Poulton, J.E

“…Biochemical changes related to cyanogenesis (hydrogen cyanide production) were monitored during maturation of black cherry (Prunus serotina Ehrh.) fruits. At…”
Get full text

Prunus serotina Amygdalin Hydrolase and Prunasin Hydrolase: Purification, N-Terminal Sequencing, and Antibody Production by Li, C P, Swain, E, Poulton, J E

“…In black cherry (Prunus serotina Ehrh.) seed homogenates, amygdalin hydrolase (AH) participates with prunasin hydrolase (PH) and mandelonitrile lyase in the…”
Get full text

Immunocytochemical localization of mandelonitrile lyase in mature black cherry (Prunus serotina Ehrh.) seeds by Wu, H C, Poulton, J E

“…Mandelonitrile lyase (MDL, EC 4.1.2.10), which catalyzes the reversible dissociation of (R)-(+)-mandelonitrile to benzaldehyde and hydrogen cyanide, was…”
Get full text

Partial purification and characterization of a 3'-phosphoadenosine 5'-phosphosulfate: desulfoglucosinolate sulfotransferase from cress (Lepidium sativum) by Glendening, T.M. (Sigma Chemical Company, St. Louis, MO), Poulton, J.E

“…A 3'-phosphoadenosine 5'-phosphosulfate (PAPS):desulfoglucosinolate sulfotransferase (EC 2.8.2-) was extensively purified from light-grown cress (Lepidium…”
Get full text

Glucosinolate biosynthesis. Sulfation of desulfobenzylglucosinolate by cell-free extracts of cress (Lepidium sativum L.) seedlings by Glendening, T.M, Poulton, J.E

“…After removal of myrosinase activity by concanavalin A-Sepharose 4B chromatography, cell-free extracts of light-grown cress (Lepidium sativum L.) seedlings,…”
Get full text

Molecular Analysis of ( R )-(+)-Mandelonitrile Lyase Microheterogeneity in Black Cherry1 by Hu, Zihua, Poulton, Jonathan E.

“…Abstract The flavoprotein (R)-(+)-mandelonitrile lyase (MDL; EC 4.1.2.10), which plays a key role in cyanogenesis in rosaceous stone fruits, occurs in black…”
Get full text

Effect of castanospermine and related polyhydroxyalkaloids on purified myrosinase from Lepidium sativum seedlings by Durham, P.L. (University of Iowa, Iowa City, IA), Poulton, J.E

“…Myrosinase (beta-thioglucoside glycohydrolase, EC 3.2.3.1) was purified to apparent homogeneity from light-grown cress (Lepidium sativum L.) seedlings. This…”
Get full text

Tissue Distributions of Dhurrin and of Enzymes Involved in Its Metabolism in Leaves of Sorghum bicolor by Mineo Kojima, Jonathan E. Poulton, Susan S. Thayer, Conn, Eric E.

“…The tissue distributions of dhurrin [p-hydroxy-(S)-mandelonitrile-β-D-glucoside] and of enzymes involved in its metabolism have been investigated in leaf…”
Get full text

Utilization of Amygdalin during Seedling Development of Prunus serotina by Swain, E., Poulton, J. E.

“…Cotyledons of mature black cherry (Prunus serotina Ehrh.) seeds contain the cyanogenic diglucoside (R)-amygdalin. The levels of amygdalin, its corresponding…”
Get full text

Catabolism of cyanogenic glycosides by purified vicianin hydrolase from squirrel's foot fern (Davallia trichomanoides Blume) by Lizotte, P.A, Poulton, J.E

“…Vicianin hydrolase, which catalyzes the hydrolysis of vicianin (Km, 4.9 millimolar) to (R)-mandelonitrile and vicianose at an optimum pH of 5.5, was…”
Get full text

Tissue level compartmentation of (R)-amygdalin and amygdalin hydrolase prevents large-scale cyanogenesis in undamaged Prunus seeds by Poulton, J.E, Li, C.P

“…Plum (Prunus domestica) seeds, which contain the cyanogenic diglucoside (R)-amygdalin and lesser amounts of the corresponding monoglucoside (R)-prunasin,…”
Get full text

Sequencing, genomic organization, and preliminary promoter analysis of a black cherry (R)-(+)-mandelonitrile lyase gene by Hu, Z, Poulton, J.E

“…The flavoprotein (R)-(+)-mandelonitrile lyase (MDL; EC 4.1.2.10) plays a key role in cyanogenesis in rosaceous stone fruits. An MDL gene (mdl3) and its…”
Get full text

Temporal and Spatial Expression of Amygdalin Hydrolase and (R)-(+)-Mandelonitrile Lyase in Black Cherry Seeds by Zheng, Liansheng, Jonathan E. Poulton

“…In black cherry (Prunus serotina Ehrh.) macerates, the cyanogenic diglucoside (R)-amygdalin undergoes stepwise degradation to HCN catalyzed by amygdalin…”
Get full text