Search Results - "Jonáková, V."
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Separation, characterization and identification of boar seminal plasma proteins
Published in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (15-04-2007)“…Methods used for the isolation, separation and characterization of boar seminal plasma proteins are discussed, as well as techniques applied to study their…”
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Localization of porcine seminal plasma (PSP) proteins in the boar reproductive tract and spermatozoa
Published in Journal of reproductive immunology (01-06-2008)“…Abstract Spermadhesins are proteins containing a characteristic CUB domain, originally isolated from seminal plasma and ejaculated spermatozoa in domestic…”
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Ubiquitin-activating enzyme (UBA1) is required for sperm capacitation, acrosomal exocytosis and sperm-egg coat penetration during porcine fertilization
Published in International journal of andrology (01-04-2012)“…Summary Protein ubiquitination is a stable, covalent post‐translational modification that alters protein activity and/or targets proteins for proteolysis by…”
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4
Origin, localization and binding abilities of boar DQH sperm surface protein tested by specific monoclonal antibodies
Published in Journal of reproductive immunology (01-06-2007)“…Abstract Seminal plasma proteins bind the sperm surface at ejaculation and may modulate several aspects of sperm activity during reproduction. DQH sperm…”
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Preliminary characterization of multiple hyaluronidase forms in boar reproductive tract
Published in Theriogenology (15-10-2007)“…Hyaluronidases play an important role in gamete interaction and fertility in mammals. The objectives of the present study were to investigate multiple forms of…”
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Spermadhesins of the AQN and AWN families, DQH sperm surface protein and HNK protein in the heparin-binding fraction of boar seminal plasma
Published in Journal of reproduction & fertility (01-09-1998)“…Heparin-binding proteins (designated BHB-2–BHB-9) were isolated from boar seminal plasma by affinity chromatography on heparin immobilized on polyacrylamide…”
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Inhibition of bacterial and boar epididymal sperm immunogenicity by boar seminal immunosuppressive component in mice
Published in Journal of reproduction & fertility (01-09-1997)“…Intravenous deposition of the immunosuppressive component, isolated from boar seminal vesicle secretion, led to suppression of primary and secondary antibody…”
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Affinity chromatography of bull seminal proteins on mannan–Sepharose
Published in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (25-11-2002)“…The interaction of bull seminal plasma proteins and sperm with mannan was investigated using an enzyme-linked binding assay (ELBA). A high mannan-binding…”
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Determination of the complete covalent structure of the major glycoform of DQH sperm surface protein, a novel trypsin-resistant boar seminal plasma O-glycoprotein related to pB1 protein
Published in Protein science (01-07-1999)“…The complete covalent structure of a novel boar DQH sperm surface protein resistant to many classical procedures of enzymatic fragmentation was determined. The…”
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10
Immobilization of l-glyceryl phosphorylcholine: isolation of phosphorylcholine-binding proteins from seminal plasma
Published in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (25-04-2002)“…The preparation of an affinity sorbent containing immobilized l-glyceryl phosphorylcholine for affinity chromatography of phosphorylcholine-binding proteins…”
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Heparin-binding proteins of human seminal plasma homologous with boar spermadhesins
Published in Journal of reproductive immunology (01-08-2001)“…Protein homologues to boar seminal plasma spermadhesins with the N-terminal sequence AQN (AQN spermadhesins) and with the N-terminal sequence AWN (AWN…”
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Inhibition of the Antibody Responses to Rat Blood Transfusion Antigens in Mice by Boar Seminal Immunosuppressive Fraction
Published in American journal of reproductive immunology (1989) (01-12-2000)“…PROBLEM: Immunosuppressive fraction of boar seminal vesicle fluid (ISF) was tested to muffle primary and secondary antibody responses to xenotranfusions…”
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13
Aggregated and monomeric forms of proteins in boar seminal plasma: characterization and binding properties
Published in Folia biologica (2000)“…Boar seminal plasma was separated into five protein fractions (I-V) (>100, 55, 45, 30, 5-15 kDa) by gel filtration chromatography on Sephadex G-75 SF at pH…”
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Reverse effect of indomethacin on the immunosuppressive activity of boar seminal immunosuppressive fraction
Published in Animal reproduction science (15-05-2002)“…The inhibitory activity of seminal immunosuppressive fraction (ISF) on mitogen-stimulated lymphocyte proliferation and on production of antibody to a soluble…”
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Aggregated forms of heparin-binding and non-heparin-binding proteins of boar seminal plasma and their binding properties
Published in Folia biologica (01-01-1999)“…Boar seminal plasma proteins were separated by affinity chromatography on immobilized heparin into two portions: heparin-binding (H+) and non-heparin-binding…”
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Water-soluble poly(acrylamide-allylamine) derivatives of saccharides for protein-saccharide binding studies
Published in Glycoconjugate journal (01-02-1995)“…Water-soluble poly(acrylamide-allylamine) copolymers containing covalently bound amino groups, prepared by copolymerization of acrylamide and allylamine, can…”
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Characterization of Boar Spermadhesins by Monoclonal and Polyclonal Antibodies and Their Role in Binding to Oocytes
Published in American journal of reproductive immunology (1989) (01-09-1999)“…PROBLEM: The role of Ala‐Trp‐Asn (AWN) and Ala‐Gln‐Asn (AQN) families of spermadhesive sperm proteins in fertilization. METHOD OF STUDY: The preparation and…”
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18
Ram proacrosin. A simple method for isolation of proacrosin free of inhibitors, and proacrosin autoactivation studies
Published in Andrologia (01-09-1984)“…Two partially purified proacrosin forms have been obtained from acid extracts of ram ejaculated spermatozoa by Sephadex G-100 column chromatography in 0.001 M…”
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D-fructose-binding proteins in bull seminal plasma: isolation and characterisation
Published in Folia biologica (01-01-2001)“…The heparin-binding activity of bull seminal plasma proteins was inhibited by D-fructose, D-glucose, inulin, and glycogen; D-galactose, dextran, and mannan had…”
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Polyvalent proteinase inhibitors from potatoes. Isolation and characterization of acrosin inhibitors from Solanum tuberosum
Published in Folia biologica (1982)“…Two trypsin inhibitor types, PII and PI II, were isolated by affinity chromatography of a potato extract on a column of trypsin immobilized on Sepharose 4B…”
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