Search Results - "Johnston, C Ian"

A novel multiplex bead-based platform highlights the diversity of extracellular vesicles by Koliha, Nina, Wiencek, Yvonne, Heider, Ute, Jüngst, Christian, Kladt, Nikolay, Krauthäuser, Susanne, Johnston, Ian C. D., Bosio, Andreas, Schauss, Astrid, Wild, Stefan

“…The surface protein composition of extracellular vesicles (EVs) is related to the originating cell and may play a role in vesicle function. Knowledge of the…”
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Ganglioside SSEA-4 in Ewing sarcoma marks a tumor cell population with aggressive features and is a potential cell-surface immune target by Jamitzky, Silke, Altvater, Bianca, Krekeler, Carolin, Hoen, Laura, Brandes, Caroline, Ebbinghaus, Julia, Richter, Lisa, Kosel, Lisa, Ochs, Laurin, Farwick, Nicole, Urban, Katja, Kluge, Lena, Bücker, Lara, Görlich, Dennis, Johnston, Ian C. D., Pfeifer, Rita, Hartmann, Wolfgang, Rossig, Claudia, Kailayangiri, Sareetha

“…Carbohydrate markers of immature cells during prenatal human development can be aberrantly expressed in cancers and deserve evaluation as immune targets. A…”
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Targeting Stage-Specific Embryonic Antigen 4 (SSEA-4) in Triple Negative Breast Cancer by CAR T Cells Results in Unexpected on Target/off Tumor Toxicities in Mice by Pfeifer, Rita, Al Rawashdeh, Wa'el, Brauner, Janina, Martinez-Osuna, Manuel, Lock, Dominik, Herbel, Christoph, Eckardt, Dominik, Assenmacher, Mario, Bosio, Andreas, Hardt, Olaf T, Johnston, Ian C D

“…Due to the paucity of targetable antigens, triple-negative breast cancer (TNBC) remains a challenging subtype of breast cancer to treat. In this study, we…”
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Prediction of cell penetrating peptides by support vector machines by Sanders, William S, Johnston, C Ian, Bridges, Susan M, Burgess, Shane C, Willeford, Kenneth O

“…Cell penetrating peptides (CPPs) are those peptides that can transverse cell membranes to enter cells. Once inside the cell, different CPPs can localize to…”
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Efficient isolation of pure and functional mitochondria from mouse tissues using automated tissue disruption and enrichment with anti-TOM22 magnetic beads by Franko, Andras, Baris, Olivier R, Bergschneider, Eva, von Toerne, Christine, Hauck, Stefanie M, Aichler, Michaela, Walch, Axel K, Wurst, Wolfgang, Wiesner, Rudolf J, Johnston, Ian C D, de Angelis, Martin Hrabĕ

“…To better understand molecular mechanisms regulating changes in metabolism, as observed e.g. in diabetes or neuronal disorders, the function of mitochondria…”
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Specific Elimination of CD133+ Tumor Cells with Targeted Oncolytic Measles Virus by BACH, Patricia, ABEL, Tobias, MÜHLEBACH, Michael D, GLIMM, Hanno, BUCHHOLZ, Christian J, HOFFMANN, Christopher, GAL, Zoltan, BRAUN, Gundula, VOELKER, Iris, BALL, Claudia R, JOHNSTON, Ian C. D, LAUER, Ulrich M, HEROLD-MENDE, Christel

“…Tumor-initiating cells (TIC) are critical yet evasive targets for the development of more effective antitumoral strategies. The cell surface marker CD133 is…”
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Generation of Antibodies Selectively Recognizing Epitopes in a Formaldehyde-Fixed Cell-Surface Antigen Using Virus-like Particle Display and Hybridoma Technology by Schatz, Stefanie, Willnow, Lena, Winkels, Monika, Rosengarten, Jamila Franca, Theek, Benjamin, Johnston, Ian C. D, Stitz, Jörn

“…Efficient induction of target-specific antibodies can be elicited upon immunization with highly immunogenic virus-like particles (VLPs) decorated with desired…”
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A Novel Siglec-4 Derived Spacer Improves the Functionality of CAR T Cells Against Membrane-Proximal Epitopes by Schäfer, Daniel, Henze, Janina, Pfeifer, Rita, Schleicher, Anna, Brauner, Janina, Mockel-Tenbrinck, Nadine, Barth, Carola, Gudert, Daniela, Al Rawashdeh, Wa'el, Johnston, Ian C D, Hardt, Olaf

“…A domain that is often neglected in the assessment of chimeric antigen receptor (CAR) functionality is the extracellular spacer module. However, several…”
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Highly Efficient and Selective CAR-Gene Transfer Using CD4- and CD8-Targeted Lentiviral Vectors by Jamali, Arezoo, Kapitza, Laura, Schaser, Thomas, Johnston, Ian C.D., Buchholz, Christian J., Hartmann, Jessica

“…Chimeric antigen receptor (CAR)-modified T cells have revealed promising results in the treatment of cancer, but they still need to overcome various hurdles,…”
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Sca‐1 expression defines developmental stages of mouse pDCs that show functional heterogeneity in the endosomal but not lysosomal TLR9 response by Niederquell, Marina, Kurig, Stefanie, Fischer, Jens A. A., Tomiuk, Stefan, Swiecki, Melissa, Colonna, Marco, Johnston, Ian C. D., Dzionek, Andrzej

“…Plasmacytoid dendritic cells (pDCs) play an important role in innate and adaptive immunity and were shown to be identical to previously described natural…”
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Characterization of HIV-1 RNA forms in the plasma of patients undergoing successful HAART by Lopez, Carlos A, Vazquez, Manuel, Hill, Martin D, Del C. Colon, Maria, Porrata-Doria, Tirtsa, Johnston, Ian C. D, Lorenzo, Eric

“…An assay to characterize plasma human immunodeficiency virus 1 (HIV-1) sequences for patients with low viral loads was developed by combining the selective…”
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Measuring enzymatic HIV-1 susceptibility to two reverse transcriptase inhibitors as a rapid and simple approach to HIV-1 drug-resistance testing by Hoffmann, Dieter, Garcia, Albert D, Harrigan, P Richard, Johnston, Ian C D, Nakasone, Tadashi, García-Lerma, J Gerardo, Heneine, Walid

“…Simple and cost-effective approaches for HIV drug-resistance testing are highly desirable for managing increasingly expanding HIV-1 infected populations who…”
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Automated manufacturing of chimeric antigen receptor T cells for adoptive immunotherapy using CliniMACS Prodigy by Mock, Ulrike, Nickolay, Lauren, Philip, Brian, Cheung, Gordon Weng-Kit, Zhan, Hong, Johnston, Ian C.D, Kaiser, Andrew D, Peggs, Karl, Pule, Martin, Thrasher, Adrian J, Qasim, Waseem

“…Highlights•In this study, we demonstrated the feasibility of CliniMACS Prodigy for T-cell transduction.•We studied the successful automated generation of…”
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Vectofusin-1 Improves Transduction of Primary Human Cells with Diverse Retroviral and Lentiviral Pseudotypes, Enabling Robust, Automated Closed-System Manufacturing by Radek, Constanze, Bernadin, Ornellie, Drechsel, Katharina, Cordes, Nicole, Pfeifer, Rita, Sträßer, Pia, Mormin, Mirella, Gutierrez-Guerrero, Alejandra, Cosset, François-Loïc, Kaiser, Andrew D, Schaser, Thomas, Galy, Anne, Verhoeyen, Els, Johnston, Ian C D

“…Cell and gene therapies are finally becoming viable patient treatment options, with both T cell- and hematopoietic stem cell (HSC)-based therapies being…”
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Specific gene transfer to neurons, endothelial cells and hematopoietic progenitors with lentiviral vectors by Buchholz, Christian J, Anliker, Brigitte, Abel, Tobias, Kneissl, Sabrina, Hlavaty, Juraj, Caputi, Antonio, Brynza, Julia, Schneider, Irene C, Münch, Robert C, Petznek, Helga, Kontermann, Roland E, Koehl, Ulrike, Johnston, Ian C D, Keinänen, Kari, Müller, Ulrike C, Hohenadl, Christine, Monyer, Hannah, Cichutek, Klaus

“…A targeting method for lentiviral vectors relying on the use of single-chain antibodies recognizing cell-surface antigens is applied to generate lentiviral…”
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HIV Nef, Paxillin, and Pak1/2 Regulate Activation and Secretion of TACE/ADAM10 Proteases by Lee, Jung-Hyun, Wittki, Sebastian, Bräu, Tanja, Dreyer, Florian S., Krätzel, Kirsten, Dindorf, Jochen, Johnston, Ian C.D., Gross, Stefanie, Kremmer, Elisabeth, Zeidler, Reinhard, Schlötzer-Schrehardt, Ursula, Lichtenheld, Mathias, Saksela, Kalle, Harrer, Thomas, Schuler, Gerold, Federico, Maurizio, Baur, Andreas S.

“…The HIV Nef protein recruits the polycomb protein Eed and mimics an integrin receptor signal for reasons that are not entirely clear. Here we demonstrate that…”
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The haemagglutinin protein is an important determinant of measles virus tropism for dendritic cells in vitro by Ohgimoto, Shinji, Ohgimoto, Kaori, Niewiesk, Stefan, Klagge, Ingo M, Pfeuffer, Joanna, Johnston, Ian C. D, Schneider-Schaulies, Jurgen, Weidmann, Armin, ter Meulen, Volker, Schneider-Schaulies, Sibylle

“…Institute for Virology and Immunobiology, University of Würzburg, Versbacher Str. 7, D-97078 Würzburg, Germany 1 Miltenyi Biotech GmbH, 51429…”
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Magselectofection: an integrated method of nanomagnetic separation and genetic modification of target cells by Sanchez-Antequera, Yolanda, Mykhaylyk, Olga, van Til, Niek P., Cengizeroglu, Arzu, de Jong, J. Henk, Huston, Marshall W., Anton, Martina, Johnston, Ian C.D., Pojda, Zygmunt, Wagemaker, Gerard, Plank, Christian

“…Research applications and cell therapies involving genetically modified cells require reliable, standardized, and cost-effective methods for cell manipulation…”
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CD44 MicroBeads accelerate HIV-1 infection in T cells by Terry, Valeri H, Johnston, Ian C.D, Spina, Celsa A

“…Abstract Super-paramagnetic CD44 MicroBeads (Miltenyi) designed for the isolation of infectious HIV-1 from dilute or difficult biological samples dramatically…”
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One-step analysis of protein complexes in microliters of cell lysate by Brock, Roland, Stoevesandt, Oda, Köhler, Karsten, Fischer, Rainer, Johnston, Ian C D

“…We present 'mix and measure' procedures for the analysis of protein complexes in microliters of crude human and mouse cell lysates using fluorescence…”
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