Search Results - "Joensson, Haakan N."

Droplet Microfluidics-A Tool for Single-Cell Analysis by Joensson, Haakan N., Andersson Svahn, Helene

“…Droplet microfluidics allows the isolation of single cells and reagents in monodisperse picoliter liquid capsules and manipulations at a throughput of…”
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Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes by Yao, Lun, Shabestary, Kiyan, Björk, Sara M., Asplund-Samuelsson, Johannes, Joensson, Haakan N., Jahn, Michael, Hudson, Elton P.

“…Cyanobacteria are model organisms for photosynthesis and are attractive for biotechnology applications. To aid investigation of genotype-phenotype…”
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High-throughput screening for industrial enzyme production hosts by droplet microfluidics by Sjostrom, Staffan L, Bai, Yunpeng, Huang, Mingtao, Liu, Zihe, Nielsen, Jens, Joensson, Haakan N, Andersson Svahn, Helene

“…A high-throughput method for single cell screening by microfluidic droplet sorting is applied to a whole-genome mutated yeast cell library yielding improved…”
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Microfluidic screening and whole-genome sequencing identifies mutations associated with improved protein secretion by yeast by Huang, Mingtao, Bai, Yunpeng, Sjostrom, Staffan L., Hallström, Björn M., Liu, Zihe, Petranovic, Dina, Uhlén, Mathias, Joensson, Haakan N., Andersson-Svahn, Helene, Nielsen, Jens

“…There is an increasing demand for biotech-based production of recombinant proteins for use as pharmaceuticals in the food and feed industry and in industrial…”
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Droplet size based separation by deterministic lateral displacement-separating droplets by cell--induced shrinking by Joensson, Haakan N, Uhlén, Mathias, Svahn, Helene Andersson

“…We present a novel method for passive separation of microfluidic droplets by size at high throughput using deterministic lateral displacement (DLD). We also…”
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A Lab-in-a-Fiber optofluidic device using droplet microfluidics and laser-induced fluorescence for virus detection by Parker, Helen E., Sengupta, Sanghamitra, Harish, Achar V., Soares, Ruben R. G., Joensson, Haakan N., Margulis, Walter, Russom, Aman, Laurell, Fredrik

“…Microfluidics has emerged rapidly over the past 20 years and has been investigated for a variety of applications from life sciences to environmental…”
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Development of a Bacterial Biosensor for Rapid Screening of Yeast p‑Coumaric Acid Production by Siedler, Solvej, Khatri, Narendar K, Zsohár, Andrea, Kjærbølling, Inge, Vogt, Michael, Hammar, Petter, Nielsen, Christian F, Marienhagen, Jan, Sommer, Morten O. A, Joensson, Haakan N

“…Transcription factor-based biosensors are used to identify producer strains, a critical bottleneck in cell factory engineering. Here, we address two challenges…”
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Multiplex analysis of enzyme kinetics and inhibition by droplet microfluidics using picoinjectors by Sjostrom, Staffan L, Joensson, Haakan N, Svahn, Helene Andersson

“…Enzyme kinetics and inhibition is important for a wide range of disciplines including pharmacology, medicine and industrial bioprocess technology. We present a…”
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Detection and Analysis of Low-Abundance Cell-Surface Biomarkers Using Enzymatic Amplification in Microfluidic Droplets by Joensson, Haakan N, Samuels, Michael L, Brouzes, Eric R, Medkova, Martina, Uhlén, Mathias, Link, Darren R, Andersson-Svahn, Helene

“…Finding the few: Cell-surface proteins are useful disease biomarkers, but current high-throughput methods are limited to detecting cells expressing more than…”
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Droplet size influences division of mammalian cell factories in droplet microfluidic cultivation by Periyannan Rajeswari, Prem Kumar, Joensson, Haakan N., Andersson‐Svahn, Helene

“…The potential of using droplet microfluidics for screening mammalian cell factories has been limited by the difficulty in achieving continuous cell division…”
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Author Correction: A Lab-in-a-Fiber optofluidic device using droplet microfluidics and laser-induced fluorescence for virus detection by Parker, Helen E., Sengupta, Sanghamitra, Harish, Achar V., Soares, Ruben R. G., Joensson, Haakan N., Margulis, Walter, Russom, Aman, Laurell, Fredrik

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Automated analysis of dynamic behavior of single cells in picoliter droplets by Khorshidi, Mohammad Ali, Rajeswari, Prem Kumar Periyannan, Wählby, Carolina, Joensson, Haakan N, Andersson Svahn, Helene

“…We present a droplet-based microfluidic platform to automatically track and characterize the behavior of single cells over time. This high-throughput assay…”
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A Portable, Negative-Pressure Actuated, Dynamically Tunable Microfluidic Droplet Generator by Trossbach, Martin, de Lucas Sanz, Marta, Seashore-Ludlow, Brinton, Joensson, Haakan N

“…Droplet microfluidics utilize a monodisperse water-in-oil emulsion, with an expanding toolbox offering a wide variety of operations on a range of droplet sizes…”
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Metabolite profiling of microfluidic cell culture conditions for droplet based screening by Bjork, Sara M, Sjostrom, Staffan L, Andersson-Svahn, Helene, Joensson, Haakan N

“…We investigate the impact of droplet culture conditions on cell metabolic state by determining key metabolite concentrations in S. cerevisiae cultures in…”
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A homogeneous assay for protein analysis in droplets by fluorescence polarization by Joensson, Haakan N., Zhang, Chi, Uhlén, Mathias, Andersson-Svahn, Helene

“…We present a novel homogeneous (“mix‐incubate‐read”) droplet microfluidic assay for specific protein detection in picoliter volumes by fluorescence…”
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Monolithic PDMS passband filters for fluorescence detection by Llobera, Andreu, Demming, Stefanie, Joensson, Haakan N, Vila-Planas, J, Andersson-Svahn, Helene, Büttgenbach, Stephanus

“…We present the fabrication and characteristics of monolithically integrated ink dyed poly(dimethylsiloxane) (PDMS) filters for optical sensing in disposable…”
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Artificial intelligence application for rapid fabrication of size-tunable PLGA microparticles in microfluidics by Damiati, Safa A., Rossi, Damiano, Joensson, Haakan N., Damiati, Samar

“…In this study, synthetic polymeric particles were effectively fabricated by combining modern technologies of artificial intelligence (AI) and microfluidics…”
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Microfluidics for cell factory and bioprocess development by Bjork, Sara M, Joensson, Haakan N

“…[Display omitted] •Microfluidic tools for bioprocess development can be categorized as top-down or bottom-up.•Microfluidic tools make new parameters accessible…”
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RNAi expression tuning, microfluidic screening, and genome recombineering for improved protein production in Saccharomyces cerevisiae by Wang, Guokun, Björk, Sara M., Huang, Mingtao, Liu, Quanli, Campbell, Kate, Nielsen, Jens, Joensson, Haakan N., Petranovic, Dina

“…The cellular machinery that supports protein synthesis and secretion lies at the foundation of cell factory-centered protein production. Due to the complexity…”
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Immune‐Modulating Mucin Hydrogel Microdroplets for the Encapsulation of Cell and Microtissue by Yan, Hongji, Melin, Marcus, Jiang, Kun, Trossbach, Martin, Badadamath, Bharathesh, Langer, Krzysztof, Winkeljann, Benjamin, Lieleg, Oliver, Hong, Jaan, Joensson, Haakan N., Crouzier, Thomas

“…Immune‐modulating biomaterials used to encapsulate cells and microtissue transplants can be engineered to dampen the immune reaction and increase treatment…”
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