Search Results - "Jin-soo Kim"

Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites by Park, Jeongbin, Bae, Sangsu, Kim, Jin-Soo

“…We present Cas-Designer, a user-friendly program to aid researchers in choosing appropriate target sites in a gene of interest for type II CRISPR/Cas-derived…”
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Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins by Kim, Sojung, Kim, Daesik, Cho, Seung Woo, Kim, Jungeun, Kim, Jin-Soo

“…RNA-guided engineered nucleases (RGENs) derived from the prokaryotic adaptive immune system known as CRISPR (clustered, regularly interspaced, short…”
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Directed evolution of CRISPR-Cas9 to increase its specificity by Lee, Jungjoon K., Jeong, Euihwan, Lee, Joonsun, Jung, Minhee, Shin, Eunji, Kim, Young-hoon, Lee, Kangin, Jung, Inyoung, Kim, Daesik, Kim, Seokjoong, Kim, Jin-Soo

“…The use of CRISPR-Cas9 as a therapeutic reagent is hampered by its off-target effects. Although rationally designed S. pyogenes Cas9 (SpCas9) variants that…”
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Cas-analyzer: an online tool for assessing genome editing results using NGS data by Park, Jeongbin, Lim, Kayeong, Kim, Jin-Soo, Bae, Sangsu

“…Genome editing with programmable nucleases has been widely adopted in research and medicine. Next generation sequencing (NGS) platforms are now widely used for…”
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Nuclear and mitochondrial DNA editing in human cells with zinc finger deaminases by Lim, Kayeong, Cho, Sung-Ik, Kim, Jin-Soo

“…Base editing in nuclear DNA and mitochondrial DNA (mtDNA) is broadly useful for biomedical research, medicine, and biotechnology. Here, we present a base…”
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Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases by Cho, Seung Woo, Kim, Sojung, Kim, Yongsub, Kweon, Jiyeon, Kim, Heon Seok, Bae, Sangsu, Kim, Jin-Soo

“…RNA-guided endonucleases (RGENs), derived from the prokaryotic adaptive immune system known as CRISPR/Cas, enable targeted genome engineering in cells and…”
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Mitochondrial DNA editing in mice with DddA-TALE fusion deaminases by Lee, Hyunji, Lee, Seonghyun, Baek, Gayoung, Kim, Annie, Kang, Beum-Chang, Seo, Huiyun, Kim, Jin-Soo

“…DddA-derived cytosine base editors (DdCBEs), composed of the split interbacterial toxin DddA tox , transcription activator-like effector (TALE), and uracil…”
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CRISPR/Cas9 searches for a protospacer adjacent motif by lateral diffusion by Globyte, Viktorija, Lee, Seung Hwan, Bae, Taegeun, Kim, Jin‐Soo, Joo, Chirlmin

“…The Streptococcus pyogenes CRISPR/Cas9 (SpCas9) nuclease has been widely applied in genetic engineering. Despite its importance in genome editing, aspects of…”
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Microhomology-based choice of Cas9 nuclease target sites by Bae, Sangsu, Kweon, Jiyeon, Kim, Heon Seok, Kim, Jin-Soo

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Adenine base editors catalyze cytosine conversions in human cells by Kim, Heon Seok, Jeong, You Kyeong, Hur, Junho K, Kim, Jin-Soo, Bae, Sangsu

“…Adenine base editors comprise an adenosine deaminase, evolved in vitro, and a Cas9 nickase. Here, we show that in addition to converting adenine to guanine,…”
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Genome-wide target specificities of CRISPR-Cas9 nucleases revealed by multiplex Digenome-seq by Kim, Daesik, Kim, Sojung, Kim, Sunghyun, Park, Jeongbin, Kim, Jin-Soo

“…We present multiplex Digenome-seq to profile genome-wide specificities of up to 11 CRISPR-Cas9 nucleases simultaneously, saving time and reducing cost…”
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Genome-wide target specificity of CRISPR RNA-guided adenine base editors by Kim, Daesik, Kim, Da-eun, Lee, Gyeorae, Cho, Sung-Ik, Kim, Jin-Soo

“…Adenine base editors 1 enable efficient targeted adenine-to-guanine single nucleotide conversions to induce or correct point mutations in human cells, animals,…”
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Florigen sequestration in cellular membranes modulates temperature-responsive flowering by Susila, Hendry, Jurić, Snježana, Liu, Lu, Gawarecka, Katarzyna, Chung, Kyung Sook, Jin, Suhyun, Kim, Soo-Jin, Nasim, Zeeshan, Youn, Geummin, Suh, Mi Chung, Yu, Hao, Ahn, Ji Hoon

“…Plants respond to temperature changes by modulating florigen activity to optimize the timing of flowering. We show that the mobile florigen FLOWERING LOCUS T…”
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Rescue of high-specificity Cas9 variants using sgRNAs with matched 5' nucleotides by Kim, Sojung, Bae, Taegeun, Hwang, Jaewoong, Kim, Jin-Soo

“…We report that engineered Cas9 variants with improved specificity-eCas9-1.1 and Cas9-HF1-are often poorly active in human cells, when complexed with single…”
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Application of Lactic Acid Bacteria (LAB) in Sustainable Agriculture: Advantages and Limitations by Raman, Jegadeesh, Kim, Jeong-Seon, Choi, Kyeong Rok, Eun, Hyunmin, Yang, Dongsoo, Ko, Young-Joon, Kim, Soo-Jin

“…Lactic acid bacteria (LAB) are significant groups of probiotic organisms in fermented food and are generally considered safe. LAB regulate soil organic matter…”
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Web-based design and analysis tools for CRISPR base editing by Hwang, Gue-Ho, Park, Jeongbin, Lim, Kayeong, Kim, Sunghyun, Yu, Jihyeon, Yu, Eunchong, Kim, Sang-Tae, Eils, Roland, Kim, Jin-Soo, Bae, Sangsu

“…As a result of its simplicity and high efficiency, the CRISPR-Cas system has been widely used as a genome editing tool. Recently, CRISPR base editors, which…”
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Efficient delivery of nuclease proteins for genome editing in human stem cells and primary cells by Liu, Jia, Gaj, Thomas, Yang, Yifeng, Wang, Nan, Shui, Sailan, Kim, Sojung, Kanchiswamy, Chidananda Nagamangala, Kim, Jin-Soo, Barbas, Carlos F

“…In this protocol, the authors describe methods for the direct delivery of ZFN, TALEN and Cas9 nuclease proteins into cells for efficient and targeted genome…”
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Direct observation of DNA target searching and cleavage by CRISPR-Cas12a by Jeon, Yongmoon, Choi, You Hee, Jang, Yunsu, Yu, Jihyeon, Goo, Jiyoung, Lee, Gyejun, Jeong, You Kyeong, Lee, Seung Hwan, Kim, In-San, Kim, Jin-Soo, Jeong, Cherlhyun, Lee, Sanghwa, Bae, Sangsu

“…Cas12a (also called Cpf1) is a representative type V-A CRISPR effector RNA-guided DNA endonuclease, which provides an alternative to type II CRISPR–Cas9 for…”
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Heritable gene knockout in Caenorhabditis elegans by direct injection of Cas9-sgRNA ribonucleoproteins by Cho, Seung Woo, Lee, Jihyun, Carroll, Dana, Kim, Jin-Soo, Lee, Junho

“…We present a novel method of targeted gene disruption that involves direct injection of recombinant Cas9 protein complexed with guide RNA into the gonad of the…”
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