Plant-derived extracts P2Et and Anamu-SC affect NO and ROS levels in leukemic cells

Plant-derived extracts P2Et and Anamu-SC affect NO and ROS levels in leukemic cells

Leukemic cells often show high nitric oxide (NO) and reactive oxygen species (ROS) levels. These can lead to resistance to apoptosis and therapy and increased proliferation. Plant-derived extracts decrease chemoresistance in cancer cells. In this study, we evaluated the effects of the plant-derived...

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Bibliographic Details
Published in:Universitas scientiarum Vol. 28; no. 2; pp. 201 - 216
Main Authors: Juan José Arévalo-Ferrin, Jimmy Alejandro García-Ortiz, Cindy Mayerli Arévalo-Olaya, Sandra Milena Quijano-Gómez, Susana Fiorentino-Gómez, Rodríguez Pardo, Viviana Marcela
Format: Journal Article
Language:English
Published: Bogotá Editorial Pontificia Universidad Javeriana 2023
Pontificia Universidad Javeriana
Subjects:
Antineoplastic drugs
Apoptosis
Caesalpinia spinosa
Cell proliferation
Cell viability
Chemoresistance
Chemotherapy
flavonoids
Flow cytometry
Fluorescence
Fluorescent indicators
Leukemia
Nitric oxide
Oxygen
Petiveria alliacea
Plant extracts
Plants
Proliferation
Reactive oxygen species
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Summary:Leukemic cells often show high nitric oxide (NO) and reactive oxygen species (ROS) levels. These can lead to resistance to apoptosis and therapy and increased proliferation. Plant-derived extracts decrease chemoresistance in cancer cells. In this study, we evaluated the effects of the plant-derived extracts P2Et (Caesalpinia spinosa) and Anamu-SC (Petiveria alliacea) and their combination with chemotherapeutic agents on NO and ROS levels in leukemic cell lines K562 and Reh. NO and ROS were determined using the DAF-FM DA and H2DCFDA probes. The mean fluorescence intensity for each variable was measured by flow cytometry. The extracts showed an antioxidant effect on both cell lines leading to a significant decrease in ROS levels without decreasing cell viability. Anamu-SC also increased NO levels in K562 cells when combined with idarubicin. Both extracts reduced the number of leukemic cells after 12 hours of treatment. Further studies are necessary to evaluate their effect on primary human leukemia cells. These findings suggest the potential of P2Et and Anamu-SC as adjuncts in leukemia treatment.
ISSN:0122-7483
2027-1352
DOI:10.11144/Javeriana.SC282.pdep