Injectable Antioxidant and Oxygen-Releasing Lignin Composites to Promote Wound Healing

The application of engineered biomaterials for wound healing has been pursued since the beginning of tissue engineering. Here, we attempt to apply functionalized lignin to confer antioxidation to the extracellular microenvironments of wounds and to deliver oxygen from the dissociation of calcium per...

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Bibliographic Details
Published in:	ACS applied materials & interfaces Vol. 15; no. 15; pp. 18639 - 18652
Main Authors:	Balaji, Swathi, Short, Walker D., Padon, Benjamin W., Belgodere, Jorge A., Jimenez, Sarah E., Deoli, Naresh T., Guidry, Anna C., Green, Justin C., Prajapati, Tanuj J., Farouk, Fayiz, Kaul, Aditya, Son, Dongwan, Jung, Olivia S., Astete, Carlos E., Kim, Myungwoong, Jung, Jangwook P.
Format:	Journal Article
Language:	English
Published:	United States American Chemical Society 19-04-2023
Subjects:	Antioxidants - pharmacology Biological and Medical Applications of Materials and Interfaces Collagen Lignin - pharmacology Oxygen Wound Healing wound healing vascularization calcium peroxide lignosulfonate reactive oxygen species
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Summary:	The application of engineered biomaterials for wound healing has been pursued since the beginning of tissue engineering. Here, we attempt to apply functionalized lignin to confer antioxidation to the extracellular microenvironments of wounds and to deliver oxygen from the dissociation of calcium peroxide for enhanced vascularization and healing responses without eliciting inflammatory responses. Elemental analysis showed 17 times higher quantity of calcium in the oxygen-releasing nanoparticles. Lignin composites including the oxygen-generating nanoparticles released around 700 ppm oxygen per day at least for 7 days. By modulating the concentration of the methacrylated gelatin, we were able to maintain the injectability of lignin composite precursors and the stiffness of lignin composites suitable for wound healing after photo-cross-linking. In situ formation of lignin composites with the oxygen-releasing nanoparticles enhanced the rate of tissue granulation, the formation of blood vessels, and the infiltration of α-smooth muscle actin+ fibroblasts into the wounds over 7 days. At 28 days after surgery, the lignin composite with oxygen-generating nanoparticles remodeled the collagen architecture, resembling the basket-weave pattern of unwounded collagen with minimal scar formation. Thus, our study shows the potential of functionalized lignin for wound-healing applications requiring balanced antioxidation and controlled release of oxygen for enhanced tissue granulation, vascularization, and maturation of collagen.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1944-8244 1944-8252
DOI:	10.1021/acsami.2c22982