The plant beneficial rhizobacterium Achromobacter sp. 5B1 influences root development through auxin signaling and redistribution

SUMMARY Roots provide physical and nutritional support to plant organs that are above ground and play critical roles for adaptation via intricate movements and growth patterns. Through screening the effects of bacterial isolates from roots of halophyte Mesquite (Prosopis sp.) on Arabidopsis thaliana...

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Published in:	The Plant journal : for cell and molecular biology Vol. 103; no. 5; pp. 1639 - 1654
Main Authors:	Jiménez‐Vázquez, Kirán R., García‐Cárdenas, Elizabeth, Barrera‐Ortiz, Salvador, Ortiz‐Castro, Randy, Ruiz‐Herrera, León F., Ramos‐Acosta, Blanca P., Coria‐Arellano, Jessica L., Sáenz‐Mata, Jorge, López‐Bucio, José
Format:	Journal Article
Language:	English
Published:	England Blackwell Publishing Ltd 01-08-2020
Subjects:	Achromobacter Achromobacter - drug effects Achromobacter - metabolism Adaptation Arabidopsis Arabidopsis - growth & development Arabidopsis - metabolism Arabidopsis - microbiology auxin signaling Bacteria Brefeldin A Brefeldin A - pharmacology Cell Division Colonization development Epidermis Gene expression Genetic analysis Growth patterns Halophytes Indoleacetic Acids - metabolism Meristem - growth & development Meristem - microbiology meristems Mesquite Mutation Organs Pin1 protein Plant growth Plant Growth Regulators - metabolism Plant nutrition Plant Roots - drug effects Plant Roots - growth & development Plant Roots - metabolism Plant Roots - microbiology Probiotics Prosopis root biology Root development Roots Seedlings Signal Transduction Signaling development meristems root biology Arabidopsis auxin signaling
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Summary:	SUMMARY Roots provide physical and nutritional support to plant organs that are above ground and play critical roles for adaptation via intricate movements and growth patterns. Through screening the effects of bacterial isolates from roots of halophyte Mesquite (Prosopis sp.) on Arabidopsis thaliana, we identified Achromobacter sp. 5B1 as a probiotic bacterium that influences plant functional traits. Detailed genetic and architectural analyses in Arabidopsis grown in vitro and in soil, cell division measurements, auxin transport and response gene expression and brefeldin A treatments demonstrated that root colonization with Achromobacter sp. 5B1 changes the growth and branching patterns of roots, which were related to auxin perception and redistribution. Expression analysis of auxin transport and signaling revealed a redistribution of auxin within the primary root tip of wild‐type seedlings by Achromobacter sp. 5B1 that is disrupted by brefeldin A and correlates with repression of auxin transporters PIN1 and PIN7 in root provasculature, and PIN2 in the epidermis and cortex of the root tip, whereas expression of PIN3 was enhanced in the columella. In seedlings harboring AUX1, EIR1, AXR1, ARF7ARF19, TIR1AFB2AFB3 single, double or triple loss‐of‐function mutations, or in a dominant (gain‐of‐function) mutant of SLR1, the bacterium caused primary roots to form supercoils that are devoid of lateral roots. The changes in growth and root architecture elicited by the bacterium helped Arabidopsis seedlings to resist salt stress better. Thus, Achromobacter sp. 5B1 fine tunes both root movements and the auxin response, which may be important for plant growth and environmental adaptation. Significance Statement Achromobacter sp. 5B1 induces Arabidopsis primary roots to deviate their growth from the gravity vector and this promotes lateral root development, thus enabling better soil exploration that accounts for enhanced plant biomass production in vitro and in soil under standard growth conditions and salt stress.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0960-7412 1365-313X
DOI:	10.1111/tpj.14853