Dynamic changes of miRNAs in skeletal muscle development at New Zealand rabbits

Dynamic changes of miRNAs in skeletal muscle development at New Zealand rabbits

miRNA is one of the crucial roles in the complex and dynamic network that regulates the development of skeletal muscle. The landscape of skeletal muscle miRNAs from fetus to adult in New Zealand rabbits has not been revealed yet. In this study, nine RNA-seq libraries of fetus, child and adult rabbit...

Full description

Saved in:
Bibliographic Details
Published in:BMC genomics Vol. 22; no. 1; pp. 1 - 577
Main Authors: Jing, Jing, Jiang, Xichun, Zhu, Cuiyun, Zheng, Qi, Ji, Qianyun, Yin, Huiqun, Huang, Jingtong, Zhu, Yixiao, Wang, Jiao, Qin, Shuaiqi, Ling, Yinghui
Format: Journal Article
Language:English
Published: London BioMed Central Ltd 27-07-2021
BioMed Central
BMC
Subjects:
Cell growth
Children
Cluster analysis
Cytoskeleton
Development
Fetuses
Genes
Genetic aspects
Genomics
Hypertrophy
Localization
Meat
Metabolism
MicroRNA
MicroRNAs
miRNA
Muscles
Musculoskeletal system
Myogenesis
Physiological aspects
Rabbit
Rabbit meat
Rabbits
Roles
Skeletal muscle
sRNA-seq
Structure
China
New Zealand
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:miRNA is one of the crucial roles in the complex and dynamic network that regulates the development of skeletal muscle. The landscape of skeletal muscle miRNAs from fetus to adult in New Zealand rabbits has not been revealed yet. In this study, nine RNA-seq libraries of fetus, child and adult rabbits' leg muscles were constructed. A total of 278 differentially expressed miRNAs (DEmiRNAs) were identified. In the fetus vs. child group, the main functional enrichments were involved in membrane and transport. Pathway enriched terms of up-regulated DEmiRNAs were connected with the differentiation and hypertrophy of skeletal muscle, and down-regulated ones were related to muscle structure and metabolic capacity. In the child vs. adult group, functions were associated to positioning and transportation, and pathways were relevant to ECM, muscle structure and hypertrophy. Finally, ocu-miR-185-3p and ocu-miR-370-3p, which had the most target genes, were identified as hub-miRNAs in these two groups. In short, we summarized the highly expressed and uniquely expressed DEmiRNAs of fetus, child and adult rabbits' leg muscles. Besides, the potential functional changes of miRNAs in two consecutive stages have been explored. Among them, the ocu-miR-185-3p and ocu-miR-370-3p with the most target genes were selected as hub-miRNAs. These data improved the understanding of the regulatory molecules of meat rabbit development, and provided a novel perspective for molecular breeding of meat rabbits.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1471-2164
1471-2164
DOI:10.1186/s12864-021-07896-5