Use of a real-time PCR method to quantify the primary infection of Plasmopara viticola in commercial vineyards

Use of a real-time PCR method to quantify the primary infection of Plasmopara viticola in commercial vineyards

Grapevine downy mildew (GDM) caused by Plasmopara viticola is a recurrent disease of wine grapes in the Ningxia Hui Autonomous Region (Ningxia) of northwestern China. However, the primary infectious pathogen in this region has not been thoroughly investigated. In this study, a multiplex real-time PC...

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Bibliographic Details
Published in:Phytopathology research Vol. 5; no. 1; pp. 1 - 13
Main Authors: Yang, Lujia, Chu, Bingyao, Deng, Jie, Yuan, Kai, Sun, Qiuyu, Jiang, Caige, Ma, Zhanhong
Format: Journal Article
Language:English
Published: London BioMed Central 06-06-2023
BMC
Subjects:
Airborne microorganisms
Asymptomatic
Correlation coefficient
Correlation coefficients
Deoxyribonucleic acid
Disease
DNA
Downy mildew
Epidemics
Epidemiology
Grapevine downy mildew
Grapevines
Infections
Inoculum
Inoculum potential
Latent
Leaves
Molecular disease index
Multiplex real-time PCR
Multiplexing
Overwintering
Pathogens
Plasmopara viticola
Polymerase chain reaction
Primary
Real time
Residues
Signs and symptoms
Vineyards
Wineries & vineyards
Wines
China
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Summary:Grapevine downy mildew (GDM) caused by Plasmopara viticola is a recurrent disease of wine grapes in the Ningxia Hui Autonomous Region (Ningxia) of northwestern China. However, the primary infectious pathogen in this region has not been thoroughly investigated. In this study, a multiplex real-time PCR assay was utilized to quantify P. viticola in soil, leaf residues, and asymptomatic leaf samples from ten commercial vineyards in two consecutive years to better understand the epidemiological significance of overwintering primary inoculum and its inoculum potential before the appearance of the first visual GDM symptoms. The DNA primers and multiplex real-time PCR assays that had been established exhibited specificity towards P. viticola within the test samples. The majority of the asymptomatic leaves (60%), leaf residues (80%), and soil samples (100%) tested positive for P. viticola . In addition, the amount of primary inoculum of P. viticola was found to be lower in soil than in leaf residues. The area under the disease progress curve in terms of the molecular disease index (AUDPC-MDI) was used to evaluate the overall latent P. viticola infection in asymptomatic leaves. Asymptomatic leaves were found to have different levels of P. viticola infection, and high AUDPC-MDIs correlated with a high AUDPC in terms of disease index (AUDPC-DI), with a significant correlation relationship between them ( P  < 0.01). Additionally, a well-correlated relationship was observed between the disease progress in the previous year and the MDIs of leaf residues and asymptomatic leaves in the following year, as well as the AUDPC-DI (Spearman’s correlation coefficient ρ = 0.643, 0.498, and 0.595, respectively) ( P  < 0.01). These findings provide valuable information for quantifying the primary infection of P. viticola in commercial vineyards.
ISSN:2524-4167
2096-5362
2524-4167
DOI:10.1186/s42483-023-00178-w