Fusion genetic analysis of gibberellin signaling mutants

Summary A fusion genetic strategy was used to identify gibberellin (GA) signaling mutants in transgenic Arabidopsis expressing the β‐glucuronidase (GUS) and firefly luciferase (LUC) reporter genes under control of the GA‐responsive GASA1 promoter. Initial analyses determined the spatial and temporal...

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Bibliographic Details
Published in:	The Plant journal : for cell and molecular biology Vol. 22; no. 5; pp. 427 - 438
Main Authors:	Raventos, Dora, Meier, Carsten, Jensen, Ole Mattsson anders B., Mundy, John
Format:	Journal Article
Language:	English
Published:	Oxford, UK Blackwell Science Ltd 01-06-2000 Blackwell Science
Subjects:	Arabidopsis thaliana Base Sequence Biological and medical sciences Cell physiology DNA Primers Fundamental and applied biological sciences. Psychology GASA1 gene Gibberellins - genetics Gibberellins - metabolism Glucuronidase - genetics GUS gene LUC gene Luciferases - genetics Luminescent Measurements Molecular and cellular biology Mutation paclobutrazol Plant Growth Regulators - pharmacology Plants, Genetically Modified Recombinant Fusion Proteins - genetics Signal transduction Signal Transduction - genetics Triazoles - pharmacology Genetic mapping Linkage Transcription promoter Gibberellin Chromosome Gene expression Arabidopsis thaliana Signal transduction Phenotype Regulation(control) Reporter gene Cruciferae Dicotyledones Angiospermae Plant growth substance Spermatophyta Transgenic plant Mutation Experimental plant Hybrid gene
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Summary:	Summary A fusion genetic strategy was used to identify gibberellin (GA) signaling mutants in transgenic Arabidopsis expressing the β‐glucuronidase (GUS) and firefly luciferase (LUC) reporter genes under control of the GA‐responsive GASA1 promoter. Initial analyses determined the spatial and temporal patterns of reporter expression, and showed that reporter induction by GA was antagonized by ABA. γ‐Irradiated M2 progeny with altered reporter activities were identified by LUC bioimaging followed by GUS assays and northern hybridization of the endogenous GASA1 mRNA. Genetic analysis showed that three mutants, which overexpressed both reporters and endogenous GASA1, were caused by recessive (goe1 and goe2, for GASA over‐expressed) and semi‐dominant (goe3) mutations at different loci. These mutants are altered in their sensitivity to GA and the GA biosynthetic inhibitor paclobutrazol, and in the expression of several GA signaling related genes.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0960-7412 1365-313X
DOI:	10.1046/j.1365-313X.2000.00759.x