Structural characterisation of a new O-methylated heteroglycan, colleman, from the cyanolichen Collema flaccidum

An alkali-extractable heteroglycan, colleman, was isolated from the cyanolichen Collema flaccidum, using ethanol fractionation and anion-exchange chromatography. The average molecular weight was estimated to be 360 kDa. Structural characterisation of the heteroglycan was performed by high-field NMR...

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Bibliographic Details
Published in:	Carbohydrate polymers Vol. 80; no. 3; pp. 799 - 807
Main Authors:	Jensen, J.S.R.E., Petersen, B.O., Veselinovic, T., Olafsdottir, E.S., Duus, J.Ø., Omarsdottir, S.
Format:	Journal Article
Language:	English
Published:	Kidlington Elsevier Ltd 05-05-2010 Elsevier
Subjects:	Applied sciences Biological and medical sciences Collema flaccidum Colleman Cyanolichen Exact sciences and technology Fundamental and applied biological sciences. Psychology Heteroglycan Microbiology Morphology, structure, chemical composition Mycology Natural polymers NMR Physicochemistry of polymers Starch and polysaccharides Heteroglycan Cyanolichen NMR Colleman Collema flaccidum Ascomycota Soxhlet extraction Molecular structure Chemical isolation Experimental study Glycan Fungi Plant origin Oside polymer
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Summary:	An alkali-extractable heteroglycan, colleman, was isolated from the cyanolichen Collema flaccidum, using ethanol fractionation and anion-exchange chromatography. The average molecular weight was estimated to be 360 kDa. Structural characterisation of the heteroglycan was performed by high-field NMR spectroscopy (1D proton, 2D-COSY, NOESY, 2D-TOCSY, 1H 13C-HSQC, HMBC, H2BC and HSQC-NOESY). According to the data obtained, the structure of colleman is composed of repeating units of A, B, C and D in approximate molar ratio 5:5:2:1; ▪
ISSN:	0144-8617 1879-1344
DOI:	10.1016/j.carbpol.2009.12.035