Embryonic--Fetal Erythroid Characteristics of a Human Leukemic Cell Line

Embryonic--Fetal Erythroid Characteristics of a Human Leukemic Cell Line

We have studied a number of cell surface, enzyme, and protein markers in the human leukemic K562 cell line. We have confirmed previous observations that these cells accumulate human embryonic hemoglobins after exposure to hemin. In addition, our results demonstrated that these cells possess in their...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS Vol. 77; no. 6; pp. 3509 - 3513
Main Authors: Benz, Edward J., Murnane, Mary J., Tonkonow, Barry L., Berman, Brian W., Mazur, Eric M., Cavallesco, Cesira, Jenko, Trina, Snyder, Edwin L., Forget, Bernard G., Hoffman, Ronald
Format: Journal Article
Language:English
Published: United States National Academy of Sciences of the United States of America 01-06-1980
National Acad Sciences
Subjects:
Antigens, Surface - analysis
B lymphocytes
Cell Differentiation
Cell Line
Cell lines
Cells
Complementary DNA
Erythrocytes
Erythrocytes - pathology
Erythroid cells
Fetal Blood
Gels
Hematopoietic Stem Cells - pathology
Hemin - pharmacology
Hemoglobins
Hemoglobins - biosynthesis
Humans
Isoenzymes
K562 cells
L-Lactate Dehydrogenase - analysis
Leukemia, Myeloid - immunology
Leukemia, Myeloid - metabolism
Leukemia, Myeloid - pathology
Messenger RNA
RNA, Messenger - analysis
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Summary:We have studied a number of cell surface, enzyme, and protein markers in the human leukemic K562 cell line. We have confirmed previous observations that these cells accumulate human embryonic hemoglobins after exposure to hemin. In addition, our results demonstrated that these cells possess in their ``uninduced'' state i surface antigen, lactate dehydrogenase isoenzymes characteristic of embryonic or fetal erythroid cells, fetal and embryonic globin chains, and globin mRNAs. The levels of i antigen, embryonic globin chains, and embryonic globin mRNA increased substantially after exposure of the cells to hemin in suspension culture. In contrast, K562 cells lacked several surface, enzymatic, and functional properties typical of granulocytes, lymphocytes, monocytes, or adult erythroblasts, including HLA surface antigens, surface immunoglobulins, sheep erythrocyte rosetting, phagocytosis, terminal deoxynucleotidyl transferase, carbonic anhydrase, ABO and Rh blood groups, and adult hemoglobins. The K562 cell line therefore exhibits phenotypic properties of embryonic erythroid progenitor cells and a quantitative increase in the expression of some of these properties can be achieved by exposure of the cells to hemin.
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content type line 23
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.77.6.3509