Search Results - "Jef D. Boeke"

CRISPR-Cas12a system in fission yeast for multiplex genomic editing and CRISPR interference by Zhao, Yu, Boeke, Jef D

“…The CRISPR-Cas12a is a class II, type V clustered regularly interspaced short palindromic repeat (CRISPR) system with both RNase and DNase activity. Compared…”
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How retrotransposons shape genome regulation by Mita, Paolo, Boeke, Jef D

“…Retrotransposons are mutagenic units able to move within the genome. Despite many defenses deployed by the host to suppress potentially harmful activities of…”
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Human Transposon Tectonics by Burns, Kathleen H., Boeke, Jef D.

“…Mobile DNAs have had a central role in shaping our genome. More than half of our DNA is comprised of interspersed repeats resulting from replicative copy and…”
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Karyotype engineering by chromosome fusion leads to reproductive isolation in yeast by Luo, Jingchuan, Sun, Xiaoji, Cormack, Brendan P., Boeke, Jef D.

“…Extant species have wildly different numbers of chromosomes, even among taxa with relatively similar genome sizes (for example, insects) 1 , 2 . This is likely…”
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Active Transposition in Genomes by Huang, Cheng Ran Lisa, Burns, Kathleen H, Boeke, Jef D

“…Transposons are DNA sequences capable of moving in genomes. Early evidence showed their accumulation in many species and suggested their continued activity in…”
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Results of Two Cases of Pig-to-Human Kidney Xenotransplantation by Montgomery, Robert A., Stern, Jeffrey M., Lonze, Bonnie E., Tatapudi, Vasishta S., Mangiola, Massimo, Wu, Ming, Weldon, Elaina, Lawson, Nikki, Deterville, Cecilia, Dieter, Rebecca A., Sullivan, Brigitte, Boulton, Gabriella, Parent, Brendan, Piper, Greta, Sommer, Philip, Cawthon, Samantha, Duggan, Erin, Ayares, David, Dandro, Amy, Fazio-Kroll, Ana, Kokkinaki, Maria, Burdorf, Lars, Lorber, Marc, Boeke, Jef D., Pass, Harvey, Keating, Brendan, Griesemer, Adam, Ali, Nicole M., Mehta, Sapna A., Stewart, Zoe A.

“…Genetically modified pig kidney xenografts were transplanted into two brain-dead human recipients. The xenografts functioned immediately and showed no evidence…”
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Widespread Transcriptional Scanning in the Testis Modulates Gene Evolution Rates by Xia, Bo, Yan, Yun, Baron, Maayan, Wagner, Florian, Barkley, Dalia, Chiodin, Marta, Kim, Sang Y., Keefe, David L., Alukal, Joseph P., Boeke, Jef D., Yanai, Itai

“…The testis expresses the largest number of genes of any mammalian organ, a finding that has long puzzled molecular biologists. Our single-cell transcriptomic…”
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Design of a synthetic yeast genome by Richardson, Sarah M., Mitchell, Leslie A., Stracquadanio, Giovanni, Yang, Kun, Dymond, Jessica S., DiCarlo, James E., Lee, Dongwon, Huang, Cheng Lai Victor, Chandrasegaran, Srinivasan, Cai, Yizhi, Boeke, Jef D., Bader, Joel S.

“…We describe complete design of a synthetic eukaryotic genome, Sc2.0, a highly modified Saccharomyces cerevisiae genome reduced in size by nearly 8%, with 1.1…”
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Affinity Proteomics Reveals Human Host Factors Implicated in Discrete Stages of LINE-1 Retrotransposition by Taylor, Martin S., LaCava, John, Mita, Paolo, Molloy, Kelly R., Huang, Cheng Ran Lisa, Li, Donghui, Adney, Emily M., Jiang, Hua, Burns, Kathleen H., Chait, Brian T., Rout, Michael P., Boeke, Jef D., Dai, Lixin

“…LINE-1s are active human DNA parasites that are agents of genome dynamics in evolution and disease. These streamlined elements require host factors to complete…”
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CREEPY: CRISPR-mediated editing of synthetic episomes in yeast by Zhao, Yu, Coelho, Camila, Lauer, Stephanie, Majewski, Miłosz, Laurent, Jon M, Brosh, Ran, Boeke, Jef D

“…Abstract Use of synthetic genomics to design and build ‘big’ DNA has revolutionized our ability to answer fundamental biological questions by employing a…”
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Synthetic Genomes by Zhang, Weimin, Mitchell, Leslie A, Bader, Joel S, Boeke, Jef D

“…DNA synthesis technology has progressed to the point that it is now practical to synthesize entire genomes. Quite a variety of methods have been developed,…”
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Sc3.0: revamping and minimizing the yeast genome by Dai, Junbiao, Boeke, Jef D., Luo, Zhouqing, Jiang, Shuangying, Cai, Yizhi

“…[...]the newly synthesized Sc3.0 chromosomes could then be combined into a single yeast to obtain strains with multiple chromosomes [8], or alternatively,…”
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Synthesis, debugging, and effects of synthetic chromosome consolidation: synVI and beyond by Mitchell, Leslie A., Wang, Ann, Stracquadanio, Giovanni, Kuang, Zheng, Wang, Xuya, Yang, Kun, Richardson, Sarah, Martin, J. Andrew, Zhao, Yu, Walker, Roy, Luo, Yisha, Dai, Hongjiu, Dong, Kang, Tang, Zuojian, Yang, Yanling, Cai, Yizhi, Heguy, Adriana, Ueberheide, Beatrix, Fenyö, David, Dai, Junbiao, Bader, Joel S., Boeke, Jef D.

“…We describe design, rapid assembly, and characterization of synthetic yeast Sc2.0 chromosome VI (synVI). A mitochondrial defect in the synVI strain mapped to…”
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Application of counter-selectable marker PIGA in engineering designer deletion cell lines and characterization of CRISPR deletion efficiency by Li, Donghui, Sun, Xiaoji, Yu, Fangzhou, Perle, Mary Ann, Araten, David, Boeke, Jef D

“…Abstract The CRISPR/Cas9 system is a technology for genome engineering, which has been applied to indel mutations in genes as well as targeted gene deletion…”
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Transcription factor profiling reveals molecular choreography and key regulators of human retrotransposon expression by Sun, Xiaoji, Wang, Xuya, Tang, Zuojian, Grivainis, Mark, Kahler, David, Yun, Chi, Mita, Paolo, Fenyö, David, Boeke, Jef D.

“…Transposable elements (TEs) represent a substantial fraction of many eukaryotic genomes, and transcriptional regulation of these factors is important to…”
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H3K36 methylation promotes longevity by enhancing transcriptional fidelity by Sen, Payel, Dang, Weiwei, Donahue, Greg, Dai, Junbiao, Dorsey, Jean, Cao, Xiaohua, Liu, Wei, Cao, Kajia, Perry, Rocco, Lee, Jun Yeop, Wasko, Brian M, Carr, Daniel T, He, Chong, Robison, Brett, Wagner, John, Gregory, Brian D, Kaeberlein, Matt, Kennedy, Brian K, Boeke, Jef D, Berger, Shelley L

“…Epigenetic mechanisms, including histone post-translational modifications, control longevity in diverse organisms. Relatedly, loss of proper transcriptional…”
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Genome-wide Consequences of Deleting Any Single Gene by Teng, Xinchen, Dayhoff-Brannigan, Margaret, Cheng, Wen-Chih, Gilbert, Catherine E, Sing, Cierra N, Diny, Nicola L, Wheelan, Sarah J, Dunham, Maitreya J, Boeke, Jef D, Pineda, Fernando J, Hardwick, J. Marie

“…Loss or duplication of chromosome segments can lead to further genomic changes associated with cancer. However, it is not known whether only a select subset of…”
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Precise control of SCRaMbLE in synthetic haploid and diploid yeast by Jia, Bin, Wu, Yi, Li, Bing-Zhi, Mitchell, Leslie A., Liu, Hong, Pan, Shuo, Wang, Juan, Zhang, Hao-Ran, Jia, Nan, Li, Bo, Shen, Michael, Xie, Ze-Xiong, Liu, Duo, Cao, Ying-Xiu, Li, Xia, Zhou, Xiao, Qi, Hao, Boeke, Jef D., Yuan, Ying-Jin

“…Compatibility between host cells and heterologous pathways is a challenge for constructing organisms with high productivity or gain of function. Designer yeast…”
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A microarray-based genetic screen for yeast chronological aging factors by Matecic, Mirela, Smith, Daniel L, Pan, Xuewen, Maqani, Nazif, Bekiranov, Stefan, Boeke, Jef D, Smith, Jeffrey S

“…Model organisms have played an important role in the elucidation of multiple genes and cellular processes that regulate aging. In this study we utilized the…”
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The Genome Project–Write by Boeke, Jef D., Church, George, Hessel, Andrew, Kelley, Nancy J., Arkin, Adam, Cai, Yizhi, Carlson, Rob, Chakravarti, Aravinda, Cornish, Virginia W., Holt, Liam, Isaacs, Farren J., Kuiken, Todd, Lajoie, Marc, Lessor, Tracy, Lunshof, Jeantine, Maurano, Matthew T., Mitchell, Leslie A., Rine, Jasper, Rosser, Susan, Sanjana, Neville E., Silver, Pamela A., Valle, David, Wang, Harris, Way, Jeffrey C., Yang, Luhan

“…We need technology and an ethical framework for genome-scale engineering The Human Genome Project (“HGP-read”), nominally completed in 2004, aimed to sequence…”
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