Cyclooxygenase-2 expression is associated with the renal macula densa of patients with Bartter-like syndrome

Cyclooxygenase-2 expression is associated with the renal macula densa of patients with Bartter-like syndrome. Bartter-like syndrome (BLS) is a heterogeneous set of congenital tubular disorders that is associated with significant renal salt and water loss. The syndrome is also marked by increased uri...

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Published in:	Kidney international Vol. 58; no. 6; pp. 2420 - 2424
Main Authors:	Kömhoff, Martin, Jeck, Nikola D.M., Seyberth, Hannsjörg W., Gröne, Hermann J., Nüsing, Rolf M., Breyer, Matthew D.
Format:	Journal Article
Language:	English
Published:	New York, NY Elsevier Inc 01-12-2000 Nature Publishing Elsevier Limited
Subjects:	Bartter Syndrome - metabolism Bartter Syndrome - pathology Biological and medical sciences Child congenital tubular disorders Cyclooxygenase 2 Dinoprostone - metabolism Gene Expression Regulation, Enzymologic Glomerulonephritis Humans In Situ Hybridization Isoenzymes - analysis Isoenzymes - genetics Juxtaglomerular Apparatus - enzymology Juxtaglomerular Apparatus - pathology Medical sciences Membrane Proteins Nephrology. Urinary tract diseases Nephropathies. Renovascular diseases. Renal failure Prostaglandin-Endoperoxide Synthases - analysis Prostaglandin-Endoperoxide Synthases - genetics prostaglandins renin Renin - metabolism Renin-Angiotensin System - physiology RNA, Messenger - analysis salt wasting urinary PGE2 excretion volume congenital tubular disorders volume prostaglandins salt wasting urinary PGE2 excretion renin Endocrinopathy Human Kidney disease Prostaglandin-endoperoxide synthase Urinary system disease Adrenal cortex diseases Hyperaldosteronism Enzyme Treatment efficiency Indometacin Enzyme inhibitor Hyperadrenocorticism Hybridization Long term Kidney Non steroidal antiinflammatory agent Bartter syndrome Pathology Biopsy Indoleacetic acid Adrenal gland diseases Oxidoreductases Child Macula densa
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Summary:	Cyclooxygenase-2 expression is associated with the renal macula densa of patients with Bartter-like syndrome. Bartter-like syndrome (BLS) is a heterogeneous set of congenital tubular disorders that is associated with significant renal salt and water loss. The syndrome is also marked by increased urinary prostaglandin E2 (PGE2) excretion. In rodents, salt and volume depletion are associated with increased renal macula densa cyclooxygenase-2 (COX-2) expression. The expression of COX-2 in human macula densa has not been demonstrated. The present studies examined whether COX-2 can be detected in macula densa from children with salt-wasting BLS versus control tissues. The intrarenal distribution of COX-2 protein and mRNA was analyzed by immunohistochemistry and in situ hybridization in 12 patients with clinically and/or genetically confirmed BLS. Renal tissue rejected for transplantation, from six adult patients not affected by BLS, was also examined. The expression of COX-2 immunoreactive protein was observed in cells of the macula densa in 8 out 11 patients with BLS. In situ hybridization confirmed the expression of COX-2 mRNA in the macula densa in 6 out of 10 cases. COX-2 protein was also detected in the macula densa in a patient with congestive heart failure. The expression of COX-2 immunoreactive protein was not observed in cells associated with the macula densa in kidneys from patients without disorders associated with hyper-reninemia. These studies demonstrate that COX-2 may be detected in the macula densa of humans. Since macula densa COX-2 was detected in cases of BLS, renal COX-2 expression may be linked to volume and renin status in humans, as well as in animals.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0085-2538 1523-1755
DOI:	10.1046/j.1523-1755.2000.00425.x