new begomovirus–betasatellite complex is associated with chilli leaf curl disease in Sri Lanka

new begomovirus–betasatellite complex is associated with chilli leaf curl disease in Sri Lanka

Leaf curl disease of chilli (LCDC) is a major constraint in production of chilli in the Indian subcontinent. The objective of this study was to identify the begomovirus species occurring in chilli in Sri Lanka, where the LCDC was initially recorded in 1938. The virus samples were collected from the...

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Bibliographic Details
Published in:Virus genes Vol. 46; no. 1; pp. 128 - 139
Main Authors: Senanayake, D. M. J. B, Jayasinghe, J. E. A. R. M, Shilpi, S, Wasala, S. K, Mandal, Bikash
Format: Journal Article
Language:English
Published: Boston Springer-Verlag 01-02-2013
Springer US
Springer Nature B.V
Subjects:
Animals
Begomovirus
Begomovirus - genetics
Begomovirus - isolation & purification
Bemisia tabaci
Biomedical and Life Sciences
Biomedicine
Capsicum - virology
Cluster Analysis
Disease Transmission, Infectious
DNA, Satellite - genetics
DNA, Satellite - isolation & purification
DNA, Viral - chemistry
DNA, Viral - genetics
Epidemics
genome
Genomes
Hemiptera - virology
leaf curling
Leaves
Lycopersicon esculentum
Medical Microbiology
Molecular Sequence Data
New species
Nucleotide sequence
nucleotides
Phylogeny
Plant Diseases - virology
Plant Sciences
Recombination
sequence analysis
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Sri Lanka
Tobacco
Tomato leaf curl Sri Lanka virus
tomatoes
Veins
Virology
viruses
Sri Lanka
India
Betasatellite
Chilli leaf curl Sri Lanka virus
Begomovirus
Sri Lanka
Chilli leaf curl
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Summary:Leaf curl disease of chilli (LCDC) is a major constraint in production of chilli in the Indian subcontinent. The objective of this study was to identify the begomovirus species occurring in chilli in Sri Lanka, where the LCDC was initially recorded in 1938. The virus samples were collected from the North Central Province, the major chilli growing region in Sri Lanka with a history of epidemic prevalence of LCDC. The virus could be readily transmitted by Bemisia tabaci to chilli, tomato and tobacco, where vein clearing followed by leaf curl developed. The genome analysis of two isolates obtained from two distantly located fields showing 100 % LCDC, revealed that the DNA-A genome (2754 nucleotides) shared 89.5 % sequence identity with each other and 68.80–84.40 % sequence identity with the other begomoviruses occurring in the Indian subcontinent. The closest identity (84.40 %) of the virus isolates was with Tomato leaf curl Sri Lanka virus (ToLCLKV). The results support that a new begomovirus species is affecting chilli in Sri Lanka and the name Chilli leaf curl Sri Lanka virus (ChiLCSLV) is proposed. Recombination analysis indicated that ChiLCSLV was a recombinant virus potentially originated from the begomoviruses prevailing in southern India and Sri Lanka. The genome of betasatellite associated with the two isolates consisted of 1366 and 1371 nucleotides and shared 95.2 % sequence identity with each other and 41.50–73.70 % sequence identity with the other betasatellite species. The results suggest that a new begomovirus betasatellite, Chilli leaf curl Sri Lanka betasatellite is associated with LCDC in Sri Lanka. This study demonstrates a new species of begomovirus and betasatellite complex is occurring in chilli in Sri Lanka and further shows that diverse begomovirus species are affecting chilli production in the Indian subcontinent.
Bibliography:http://dx.doi.org/10.1007/s11262-012-0836-4
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ISSN:0920-8569
1572-994X
DOI:10.1007/s11262-012-0836-4