Search Results - "Jayasena, Sumedha"

Functional siRNAs and miRNAs Exhibit Strand Bias by Khvorova, Anastasia, Reynolds, Angela, Jayasena, Sumedha D.

“…Both microRNAs (miRNA) and small interfering RNAs (siRNA) share a common set of cellular proteins (Dicer and the RNA-induced silencing complex [RISC]) to…”
Get full text

Aptamers: An Emerging Class of Molecules That Rival Antibodies in Diagnostics by Jayasena, Sumedha D

“…Antibodies, the most popular class of molecules providing molecular recognition needs for a wide range of applications, have been around for more than three…”
Get full text

Fast Cleavage Kinetics of a Natural Hammerhead Ribozyme by Canny, Marella D, Jucker, Fiona M, Kellogg, Elizabeth, Khvorova, Anastasia, Jayasena, Sumedha D, Pardi, Arthur

“…The hammerhead ribozyme is a small RNA motif that catalyzes the cleavage and ligation of RNA. The well-studied minimal hammerhead motif is inactive under…”
Get full text

Folding of the natural hammerhead ribozyme is enhanced by interaction of auxiliary elements by Penedo, J Carlos, Wilson, Timothy J, Jayasena, Sumedha D, Khvorova, Anastasia, Lilley, David M J

“…It has been shown that the activity of the hammerhead ribozyme at microM magnesium ion concentrations is markedly increased by the inclusion of loops in…”
Get full text

RNA Interference Blocks Gene Expression and RNA Synthesis from Hepatitis C Replicons Propagated in Human Liver Cells by Wilson, Joyce A., Jayasena, Sumedha, Khvorova, Anastasia, Sabatinos, Sarah, Rodrigue-Gervais, Ian Gaël, Arya, Sudha, Sarangi, Farida, Harris-Brandts, Marees, Beaulieu, Sylvie, Richardson, Christopher D.

“…RNA interference represents an exciting new technology that could have therapeutic applications for the treatment of viral infections. Hepatitis C virus (HCV)…”
Get full text

Artificial tertiary motifs stabilize trans-cleaving hammerhead ribozymes under conditions of submillimolar divalent ions and high temperatures by Saksmerprome, Vanvimon, Roychowdhury-Saha, Manami, Jayasena, Sumedha, Khvorova, Anastasia, Burke, Donald H

“…Tertiary stabilizing motifs (TSMs) between terminal loops or internal bulges facilitate folding of natural hammerhead ribozymes (hRz) under physiological…”
Get full text

Inhibition of multiple thermostable DNA polymerases by a heterodimeric aptamer by Lin, Yun, Jayasena, Sumedha D

“…Single-stranded DNA aptamers that recognize DNA polymerase from Thermus acquaticus ( Taq pol) with high affinity have been described recently. These aptamers…”
Get full text

Signal amplification through nucleotide extension and excision on a dendritic DNA platform by Capaldi, S, Getts, R C, Jayasena, S D

“…Techniques that provide strong signal amplification are useful in diagnostic applications, especially in detecting low concentrations of non-amplifiable target…”
Get full text

Functional siRNAs and miRNAs Exhibit Strand Bias by Khvorova, Anastasia, Reynolds, Angela, Jayasena, Sumedha D.

Get full text

Peptide Conjugation to an in vitro-Selected DNA Ligand Improves Enzyme Inhibition by Lin, Yun, Padmapriya, Abeysinghe, Morden, Kathleen M., Jayasena, Sumedha D.

“…An in vitro selection technique was used to identify a specific high-affinity DNA ligand targeted to human neutrophil elastase (HNE).1H NMR data and a…”
Get full text

Sequence elements outside the hammerhead ribozyme catalytic core enable intracellular activity by Khvorova, Anastasia, Lescoute, Aurélie, Westhof, Eric, Jayasena, Sumedha D

“…The hammerhead ribozyme (HHRz) is a small, naturally occurring ribozyme that site-specifically cleaves RNA and has long been considered a potentially useful…”
Get full text

Acceleration of Diabetic Wound Healing with PHD2- and miR-210-Targeting Oligonucleotides by Dallas, Anne, Trotsyuk, Artem, Ilves, Heini, Bonham, Clark A, Rodrigues, Melanie, Engel, Karl, Barrera, Janos A, Kosaric, Nina, Stern-Buchbinder, Zachary A, White, Aleksandr, Mandell, Kenneth J, Hammond, Paula T, Mansbridge, Jonathan, Jayasena, Sumedha, Gurtner, Geoffrey C, Johnston, Brian H

“…In diabetes-associated chronic wounds, the normal response to hypoxia is impaired and many cellular processes involved in wound healing are hindered. Central…”
Get more information

Sequence limitations of triple helix formation by alternate-strand recognition by Jayasena, Sumedha D, Johnston, Brian H

“…Until recently, oligonucleotide-directed triplex formation has been limited to oligopurine tracts of target DNA. Triplex formation by alternate-strand…”
Get full text

Intramolecular triple-helix formation at (PunPyn).cntdot.(PunPyn) tracts: recognition of alternate strands via Pu.cntdot.PuPy and Py.cntdot.PuPy base triplets by Jayasena, Sumedha D, Johnston, Brian H

Get full text

Site-Specific Cleavage of the Transactivation Response Site of Human Immunodeficiency Virus RNA with a Tat-Based Chemical Nuclease by Jayasena, Sumedha D., Johnston, Brian H.

“…tat, an essential transactivator of gene transcription in the human immunodeficiency virus (HIV), is believed to activate viral gene expression by binding to…”
Get full text

Nucleosome reconstitution of core-length poly(dG.cntdot.poly(dC) and poly(rG-dC).cntdot.poly(rG-dC) by Jayasena, Sumedha D, Behe, Michael J

Get full text

Staining of cell surface human CD4 with 2′-F-pyrimidine-containing RNA aptamers for flow cytometry by Davis, Kenneth A., Abrams, Barnaby, Lin, Yun, Jayasena, Sumedha D.

“…We have used recombinant human CD4 presented on beads as an affinity matrix to screen a 2′-F-pyrimidinecontaining RNA library with a complexity of ∼1014…”
Get full text

Oligonucleotide inhibitors of Taq DNA polymerase facilitate detection of low copy number targets by PCR by Dang, C, Jayasena, S D

“…A random sequence library of single stranded DNA was screened to isolate sequences with high affinity for Thermus aquaticus DNA polymerase (Taq pol), a…”
Get full text

Modified RNA sequence pools for in vitro selection by Lin, Yun, Qiu, Qiu, Gill, Stanley C., Jayasena, Sumedha D.

“…We report the use of modified RNA, In which the 2′-OH group of pyrimldlnes is replaced by a 2′-amino (2′-NH2) group to Identify high affinity ligands specific…”
Get full text

OligonucleotJde-directed triple helix formation at adjacent oligopurine and oligopyrimidine DNA tracts by alternate strand recognition by Jayasena, Sumedha D., Johnston, Brian H.

“…A significant limitation to the practical application of triplex DNA is its requirement for oligopurine tracts in target DNA sequences. The repertoire of…”
Get full text