Protocol to produce plant-based hybrid nanovesicles from fresh turmeric and pepper using polyethylene glycol

Protocol to produce plant-based hybrid nanovesicles from fresh turmeric and pepper using polyethylene glycol

In addition to proteins, microRNAs, and lipids, plant-derived exosome-like nanovesicles (ENVs) are also enriched with host plant bioactives. Both curcumin and piperine are water insoluble, lack bioavailability, and are extracted by non-ecofriendly solvents. Herein, we present an eco-friendly protoco...

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Published in:STAR protocols Vol. 5; no. 1; p. 102924
Main Authors: Kumar, Meghana N., Kalarikkal, Sreeram Peringattu, Jayaram, Yashaswini, Narayanan, Janakiraman, Sundaram, Gopinath M.
Format: Journal Article
Language:English
Published: United States Elsevier Inc 15-03-2024
Elsevier
Subjects:
Alkaloids
Benzodioxoles
Cell Membrane
Curcuma
Curcumin
Environmental sciences
Health Sciences
Piperidines
Plant sciences
Polyethylene Glycols
Polyunsaturated Alkamides
Protocol
Cell Membrane
Plant sciences
Environmental sciences
Health Sciences
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Summary:In addition to proteins, microRNAs, and lipids, plant-derived exosome-like nanovesicles (ENVs) are also enriched with host plant bioactives. Both curcumin and piperine are water insoluble, lack bioavailability, and are extracted by non-ecofriendly solvents. Herein, we present an eco-friendly protocol for co-isolating both curcumin and piperine in the form of hybrid ENVs. We describe steps for sample pre-processing, combined homogenization of plant materials, filtration, and differential centrifugation. We then detail procedures for polyethylene glycol-based fusion and precipitation of hybrid ENVs. For complete details on the use and execution of this protocol, please refer to Kumar et al.1 [Display omitted] •Protocol for the purification of both curcumin and piperine in hybrid nanovesicles•One-pot preparation of hybrid nanovesicles (TPENVs) from edible plants•Obtain synergistic bioactives from different plant species in one protocol Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. In addition to proteins, microRNAs, and lipids, plant-derived exosome-like nanovesicles (ENVs) are also enriched with host plant bioactives. Both curcumin and piperine are water insoluble, lack bioavailability, and are extracted by non-ecofriendly solvents. Herein, we present an eco-friendly protocol for co-isolating both curcumin and piperine in the form of hybrid ENVs. We describe steps for sample pre-processing, combined homogenization of plant materials, filtration, and differential centrifugation. We then detail procedures for polyethylene glycol-based fusion and precipitation of hybrid ENVs.
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ISSN:2666-1667
2666-1667
DOI:10.1016/j.xpro.2024.102924