Characterization of Quercus species distributed in Jordan using morphological and molecular markers

Genetic diversity among 25 natural populations of three different species of Quercus in Jordan at morphological and molecular levels using random amplified polymorphic DNA (RAPD) primers was assessed. Significant morphological and molecular variations among and within 25 Quercus populations were est...

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Bibliographic Details
Published in:	African journal of biotechnology Vol. 12; no. 12; pp. 1326 - 1334
Main Authors:	Jawarneh, MS, Brake, M H, Muhaidat, R, Migdadi, H M, Lahham, J N, El-Oqlah, A A
Format:	Journal Article
Language:	English
Published:	20-03-2013
Subjects:	Quercus
Online Access:	Get full text
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Summary:	Genetic diversity among 25 natural populations of three different species of Quercus in Jordan at morphological and molecular levels using random amplified polymorphic DNA (RAPD) primers was assessed. Significant morphological and molecular variations among and within 25 Quercus populations were estimated. Standardized canonical discrimination functions for the investigated morphological traits showed that the first function explains 72.44% of the total variability between populations and was strongly influenced by leaf and scale length. Twenty-seven polymorphic markers and 5917 scored bands were generated using six RAPD primers. Based on morphological and RAPD data, the populations were grouped together in the same cluster according to species regardless of local of collections. Result of cluster analysis based on RAPD showed significant correlation with morphological characters based on Mantel's test (r = 0.145**). This study has emphasized the ability of the morphological and molecular markers in determining the genetic diversity among and within the populations of Quercus and that the resulted high genetic variability could be utilized in implications of improving conservation, restoration and reforestation strategies of Quercus in Jordan.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 content type line 23 ObjectType-Feature-2
ISSN:	1684-5315 1684-5315
DOI:	10.5897/AJB12.2344