Degradation efficiency of phenolic compounds using immobilized peroxidase purified from soybean
This study was aimed on purification, immobilization and application of peroxidase enzyme from whole plant of soy bean for the decolorization of textile dyes and degradation of some phenolic compounds. The purification of peroxidase was in two steps include: concentration by sucrose and gel filtrati...
Saved in:
| Published in: | Iraqi journal of agricultural science Vol. 50; no. 3; pp. 928 - 935 |
|---|---|
| Main Authors: | , |
| Format: | Journal Article |
| Language: | Arabic English |
| Published: |
Baghdad, Iraq
University of Baghdad, College of Agriculture
01-01-2019
|
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | This study was aimed on purification, immobilization and application of peroxidase enzyme
from whole plant of soy bean for the decolorization of textile dyes and degradation of some
phenolic compounds. The purification of peroxidase was in two steps include: concentration
by sucrose and gel filtration by using sephadex G-150. The purification fold was 4.46 and 2.09
with an enzyme yield of 22.34% and 25.55% for peak 1 and peak 2 respectively. The purified
peroxidase enzyme was immobilized by two methods include: covalent linkage immobilization
by gluteraldehyde activated chitosan and entrapment by sodium alginate and agarose gel, the
immobilization ratio reached to 58.07%, 50% and 11.75% respectively. Crude, purified and
immobilized peroxidase were studied with textile dyes (red, blue, yellow and black) after 24
hr, the maximum removal efficiency was with immobilized peroxidase reached to 13.25, 35.12,
14.37 and 26.92 % respectively. Immobilized peroxidase on chitosan was able to degrade some
phenolic compounds (tannic acid, naphthalene and gallic acid) during 2 hr, and the
degradation efficiency was reached to 95.85%, 79.75% and 33.88% respectively. |
|---|---|
| ISSN: | 0075-0530 2410-0862 |
| DOI: | 10.36103/ijas.v50i3.709 |