Application of tail vein serial microsampling for plasma or dried plasma spots in toxicokinetic assessment in rats using acetaminophen as the model compound

In the current study, two groups of rats (five per group) were administered a single oral dose of 500 mg/kg acetaminophen. For toxicokinetic assessment, the Group 1 animals were bled via conventional sparse (two animals/time point) sublingual vein bleeding (~0.5 ml) with anesthesia, while the Group...

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Bibliographic Details
Published in:	Biomedical chromatography Vol. 34; no. 10; pp. e4917 - n/a
Main Authors:	Li, Wenkui, Dugyala, Ravi, Devine, Patrick J., Jardel, Matthew, Doherty, John, Kubek, Katie, Lapadula, Daniel, Flarakos, Jimmy
Format:	Journal Article
Language:	English
Published:	England 01-10-2020
Subjects:	acetaminophen Acetaminophen - blood Acetaminophen - chemistry Acetaminophen - toxicity Animals Blood Specimen Collection - adverse effects Blood Specimen Collection - methods Chromatography, Liquid Corticosterone - blood Dried Blood Spot Testing - methods dried plasma spots (DPS) Male microsampling Models, Chemical Rats rodent toxicokinetic studies sparse sampling Stress, Psychological Tail - blood supply Tandem Mass Spectrometry Toxicokinetics rodent toxicokinetic studies sparse sampling acetaminophen dried plasma spots (DPS) microsampling
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Summary:	In the current study, two groups of rats (five per group) were administered a single oral dose of 500 mg/kg acetaminophen. For toxicokinetic assessment, the Group 1 animals were bled via conventional sparse (two animals/time point) sublingual vein bleeding (~0.5 ml) with anesthesia, while the Group 2 animals were bled via serial tail vein microsampling (~0.075 ml) without anesthesia. All collected blood was processed for plasma. Each Group 2 plasma sample (~30 μl) was divided into ‘wet’ and ‘dried’ (dried plasma spots). All plasma samples were analyzed by LC–MS/MS for acetaminophen and its major metabolites acetaminophen glucuronide and acetaminophen sulfate. In addition, plasma and urine samples were collected for analysis of corticosterone and creatinine to assess stress levels. Comparable plasma exposure to acetaminophen and its two metabolites was observed in the plasma obtained via conventional sparse sublingual vein bleeding and serial tail vein microsampling and between the ‘wet’ and ‘dried’ plasma obtained by the latter. Furthermore, comparable corticosterone levels or corticosterone/creatinine ratios between the two groups suggested that serial microsampling without anesthesia did not increase the levels of stress as compared with conventional sampling with anesthesia, confirming the utility of microsampling for plasma or dried plasma spots in rodent toxicokinetic assessment.
Bibliography:	Current address: Toxicology, Arbutus Biopharma Corporation, 701 Veterans Circle, Warminster, PA 18974, USA. Current address: LC/MS Bioanalysis, Pharmaron, 20340 Seneca Meadows Parkway, Germantown, MD 20876, USA.
ISSN:	0269-3879 1099-0801
DOI:	10.1002/bmc.4917