A rapid and easy method for the DNA extraction from Cryptococcus neoformans

DNA isolation from C. neoformans is difficult due to a thick and resistant capsule. We have optimized a new and rapid DNA isolation method for Cryptococcus using a short urea treatment followed by a rapid method using a chelex resin suspension. This procedure is simpler than previously reported meth...

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Bibliographic Details
Published in:	Biological procedures online Vol. 13; no. 1; p. 5
Main Authors:	Mseddi, Fatma, Jarboui, Mohammed Ali, Sellami, Amira, Sellami, Hayet, Ayadi, Ali
Format:	Journal Article
Language:	English
Published:	England BioMed Central Ltd 21-07-2011 BioMed Central BMC
Subjects:	Cryptococcus Physiological aspects Polymerase chain reaction Urea Tunisia
Online Access:	Get full text
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Summary:	DNA isolation from C. neoformans is difficult due to a thick and resistant capsule. We have optimized a new and rapid DNA isolation method for Cryptococcus using a short urea treatment followed by a rapid method using a chelex resin suspension. This procedure is simpler than previously reported methods.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1480-9222 1480-9222
DOI:	10.1186/1480-9222-13-5