pPCV, a versatile vector for cloning PCR products

The efficiency of PCR product cloning depends on the nature of the DNA polymerase employed because amplicons may have blunt-ends or 3′ adenosines overhangs. Therefore, for amplicon cloning, available commercial vectors are either blunt-ended or have a single 3′ overhanging thymidine. The aim of this...

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Bibliographic Details
Published in:	SpringerPlus Vol. 2; no. 1; p. 441
Main Authors:	Janner, Christiane R, Brito, Ana Lívia P, Moraes, Lidia Maria P, Reis, Viviane CB, Torres, Fernando AG
Format:	Journal Article
Language:	English
Published:	Cham Springer International Publishing 01-01-2013 Springer Nature B.V
Subjects:	Biomedical and Life Sciences Humanities and Social Sciences multidisciplinary Science Science (multidisciplinary) Polymerase chain reaction Plasmid Molecular cloning
Online Access:	Get full text
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Summary:	The efficiency of PCR product cloning depends on the nature of the DNA polymerase employed because amplicons may have blunt-ends or 3′ adenosines overhangs. Therefore, for amplicon cloning, available commercial vectors are either blunt-ended or have a single 3′ overhanging thymidine. The aim of this work was to offer in a single vector the ability to clone both types of PCR products. For that purpose, a minimal polylinker was designed to include restriction sites for Eco RV and Xcm I which enable direct cloning of amplicons bearing blunt-ends or A-overhangs, respectively, still offering blue/white selection. When tested, the resulting vector, pPCV, presented high efficiency cloning of both types of amplicons.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2193-1801 2193-1801
DOI:	10.1186/2193-1801-2-441