Endometritis Increases Pro-inflammatory Cytokines in Follicular Fluid and Cervico-vaginal Mucus in the Buffalo Cow

Endometritis Increases Pro-inflammatory Cytokines in Follicular Fluid and Cervico-vaginal Mucus in the Buffalo Cow

Emerging evidence shows that some of the pro-inflammatory cytokines are elevated not only in the endometrium but also in the follicular fluid of cows with endometritis. Developing a cervico-vaginal mucus (CVM) based test has the potential for becoming a pen-side test because of the ease of sample co...

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Bibliographic Details
Published in:Animal biotechnology Vol. 28; no. 3; pp. 163 - 167
Main Authors: Boby, Jones, Kumar, Harendra, Gupta, Harihar Prasad, Jan, Mustapha Hussain, Singh, Sanjay Kumar, Patra, Manas Kumar, Nandi, Sukdeb, Abraham, Asha, Krishnaswamy, Narayanan
Format: Journal Article
Language:English
Published: England Taylor & Francis 03-07-2017
Taylor & Francis Ltd
Subjects:
17β-Estradiol
Animals
Buffalo
Buffaloes
Cattle
Cattle Diseases - metabolism
cervico-vaginal mucus
Cervix Mucus - chemistry
Cervix Mucus - metabolism
Cytokines
Cytokines - analysis
Cytokines - metabolism
Cytology
Diagnostic systems
Endometritis
Endometritis - metabolism
Endometritis - veterinary
Endometrium
Enzyme-linked immunosorbent assay
Estradiol - analysis
Estradiol - metabolism
Female
Follicular fluid
Follicular Fluid - metabolism
Genitalia
Inflammation
Interleukin 1
Interleukin 6
Interleukin 8
Mucus
Progesterone - analysis
Progesterone - metabolism
proinflammatory cytokine
Repeaters
Sex hormones
Slaughter
Steroidogenesis
Tumor necrosis factor
Vagina
Vagina - chemistry
Vagina - metabolism
cervico-vaginal mucus
proinflammatory cytokine
endometritis
Buffaloes
follicular fluid
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Summary:Emerging evidence shows that some of the pro-inflammatory cytokines are elevated not only in the endometrium but also in the follicular fluid of cows with endometritis. Developing a cervico-vaginal mucus (CVM) based test has the potential for becoming a pen-side test because of the ease of sample collection. The present study describes the results of two different experiments. The first experiment was conducted to investigate the influence of endometritis on the proinflammatory cytokines of follicular fluid based on the reproductive tracts of buffalo collected at a slaughter house Buffalo genitalia were categorized into purulent endometritis (PE), cytological endometritis (CE), and non-endometritis (NE) based on the white-side test and endometrial cytology, respectively (n = 14/group). Each group was subdivided into follicular and mid-luteal stage (n = 7/stage) and the follicular fluid was collected from the largest follicle. Second experiment was done to study the difference in the levels of proinflammatory cytokines in the CVM of repeat breeders with subclinical endometritis presented to the clinic. CVM was collected from the repeaters (n = 10) and non-repeaters (n = 10) through aseptic trans-vaginal aspiration. The pro-inflammatory cytokines such as IL-1β, IL-6, IL-8, and TNFα were quantitated through bovine specific ELISA kits. Significantly higher concentrations of pro-inflammatory cytokines (IL-1β, IL-8, IL-6, and TNFα) along with low intra-follicular estradiol in buffaloes of PE and CE groups suggest that endometritis impedes the follicular steroidogenesis. Significantly higher concentration of IL-1β and TNF-α in the CVM of repeaters indicate their potential as a pen-side diagnostic test for CE.
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ISSN:1049-5398
1532-2378
DOI:10.1080/10495398.2016.1244067