A putative heat-responsive transcription factor (TaHD97) and its targets in wheat (Triticum aestivum) providing thermotolerance

A putative heat-responsive transcription factor (TaHD97) and its targets in wheat (Triticum aestivum) providing thermotolerance

Transcription factors (TFs) are protein, which perform their role at transcriptional level by affecting the expression of various genes associated with metabolic pathways, growth and stress-associated genes (SAGs) at different developmental stages. Here, we identified 38 novel heat-responsive transc...

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Bibliographic Details
Published in:Indian journal of biotechnology Vol. 18; no. 3; p. 214
Main Authors: Singh, J P, Kumar, R Kumar, Goswami, S, Rai, G K, Sakhare, A, Kumar, S, Singh, S D, Bakshi, S, Jamhulkar, S J, Sareen, Sindhu, Singh, G P, Chinnusamy, V, Praveen, S
Format: Journal Article
Language:English
Published: New Delhi National Institute of Science Communication & Information Resources 01-07-2019
Subjects:
Amino acid sequence
Amino acids
Deoxyribonucleic acid
Developmental stages
DNA
Gene expression
Genes
Heat shock
Heat stress
Heat tolerance
Hsp90 protein
Metabolic pathways
Nucleotide sequence
Temperature tolerance
Tissue analysis
Transcription factors
Wheat
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Summary:Transcription factors (TFs) are protein, which perform their role at transcriptional level by affecting the expression of various genes associated with metabolic pathways, growth and stress-associated genes (SAGs) at different developmental stages. Here, we identified 38 novel heat-responsive transcription factor genes from wheat cv. HD2985 by mining the de novo transcriptome data derived from heat shock (HS) treated wheat. Based on digital gene expression (DGE), a putative transcript (TaHD97) of ~1.1 kbas amplified and cloned from wheat cv. HD2985. The presence of heat stress transcription factor (HSF) DNA binding domain was observed in the amino acid sequence. Differential expression of TaHD97 was observed in HD2985 (thermotolerant) and HD2329 (thermosensitive) under heat stress. Tissue specific expression analysis showed up-regulation of TaHD97 in leaves, stem and endospermic tissues and down-regulation in root under HS. A positive correlation was established between the expression of TaHD97 and its target gene (HSP17 and HSP90) in wheat under heat stress. HSP17 transcripts were observed more in leaves of HD2985, as compared to HD2329. Thermotolerance related biochemical enzymes (SOD, CAT, GPX and TBARS) were observed higher in wheat cv. HD2985 showing maximum expression of TaHD97 under heat stress. There is a need for the functional validation of the gene TaHD97 in order to use it for the regulation of sHSP (catalytic chaperone) - a novel approach towards augmenting thermotolerance in wheat under heat stress.
ISSN:0972-5849
0975-0967