Soluble plasma antigen in experimental Salmonella typhimurium infection in mice

To detect and characterize Salmonella antigen in blood, outbred CF‐1 female mice were inoculated intraperitoneally with S. typhimurium LT‐2 and blood was assayed by ELISA for Salmonella common structural antigen. Plasma antigen was detectable early in the course of infection and increased in quantit...

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Bibliographic Details
Published in:	FEMS immunology and medical microbiology Vol. 9; no. 4; pp. 325 - 332
Main Authors:	Butler, Thomas, Rolfe, Rial D., James, Gina Marie, Hentges, David J.
Format:	Journal Article
Language:	English
Published:	Oxford, UK Blackwell Publishing Ltd 01-10-1994 Blackwell
Subjects:	Animals Antigenema Antigens, Bacterial - blood Bacterial diseases Biological and medical sciences Chromatography, Gel Enzyme-Linked Immunosorbent Assay Enzyme‐linked immunoassay Experimental bacterial diseases and models Female Hot Temperature Infectious diseases Medical sciences Mice Salmonella Infections, Animal - immunology Salmonella typhimurium Salmonella typhimurium - immunology Animal model Molecular weight determination Rodentia Soluble form Typhoid Salmonella typhimurium Blood Infection Antigen Vertebrata Experimental disease Mammalia Mouse Bacteriosis Bacteria Salmonellosis Detection ELISA assay Enterobacteriaceae
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Summary:	To detect and characterize Salmonella antigen in blood, outbred CF‐1 female mice were inoculated intraperitoneally with S. typhimurium LT‐2 and blood was assayed by ELISA for Salmonella common structural antigen. Plasma antigen was detectable early in the course of infection and increased in quantity later in the course of illness when animals showed high grade bacteremia and high counts of splenic bacteria. Antigen was associated with a cell‐free plasma fraction of blood, passed through filters with cut‐offs of 0.2 μ and molecular mass of 1000 kDa, and was enhanced in detectability after heating to 100°C for 15 min. Antigen was concentrated by diluting plasma 1:4 in 0.1 M EDTA, heating to 100°C, and concentrating the supernate with an ultrafiltration membrane with a molecular mass cut‐off of 15 kDa. By gel filtration, antigen was associated with a peak at about molecular mass 300 kDa in heated plasma and a peak at about 380 kDa in unheated plasma. These results indicate that murine typhoid infection results in circulating soluble plasma antigen, which is heat‐stable with a molecular mass of approximately 300 kDa.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0928-8244 1574-695X
DOI:	10.1111/j.1574-695X.1994.tb00368.x