Green Synthesis of Silver Nanoparticles from Aqueous Extract of Tinospora crispa Stems Accelerate Wound Healing in Rats

Green Synthesis of Silver Nanoparticles from Aqueous Extract of Tinospora crispa Stems Accelerate Wound Healing in Rats

Tinospora crispa has been known as an herbal medicine with many biological activities. The current study evaluates silver nanoparticles in accelerating the rate of wound healing and closure of excision wounds in rats. A novel technique was followed to synthesize nanosilver particles, x-ray diffracti...

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Bibliographic Details
Published in:International journal of lower extremity wounds p. 15347346221133627
Main Authors: Osman Mahmud, Shokhan, Hamad Shareef, Suhayla, Jabbar, Ahmed A. J., Hassan, Rawaz Rizgar, Jalal, Hardy Khalid, Abdulla, Mahmood Ameen
Format: Journal Article
Language:English
Published: Los Angeles, CA SAGE Publications 03-11-2022
Subjects:
silver nanoparticle
wound
Tinospora crispa
immunohistochemistry
histology
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Summary:Tinospora crispa has been known as an herbal medicine with many biological activities. The current study evaluates silver nanoparticles in accelerating the rate of wound healing and closure of excision wounds in rats. A novel technique was followed to synthesize nanosilver particles, x-ray diffraction, UV–Vis's spectroscopy, and transition electron microscope were used. Sprague Dawley rats (24) were separated into 4 clusters: vehicle group (G1), treated with Vaseline; positive control (G2), treated with the intrasite gel; Experimental groups (G3 and G4), treated with 175 and 350 ppm silver nanoparticles, respectively, with an excisional wound created on the neck area. Wounds were treated topically twice daily for two weeks and sacrificed on day fifteenth. Histology, immunohistochemistry (expression of Bax and HSP 70 proteins), and Masson Trichrome staining procedures disclose that wounds dressed with silver nanoparticles exhibited wound closure site contained less scar, less inflammatory cells, and more collagen compared vehicle group. The endogenous antioxidant enzymes (SOD and CAT) and TGF-β were significantly increased and lipid peroxidation (MDA) and TNF-α were decreased in wound tissue homogenate dressed with silver nanoparticles compared to the vehicle cluster. Silver nanoparticles suggestively improved cell proliferation and stimulated the rate of wound closure, with less scarring, and more fibroblast. Graphical abstract
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ISSN:1534-7346
1552-6941
DOI:10.1177/15347346221133627