Rice EARLY SENESCENCE 2, encoding an inositol polyphosphate kinase, is involved in leaf senescence

Early leaf senescence influences yield and yield quality by affecting plant growth and development. A series of leaf senescence-associated molecular mechanisms have been reported in rice. However, the complex genetic regulatory networks that control leaf senescence need to be elucidated. In this stu...

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Published in:	BMC plant biology Vol. 20; no. 1; pp. 393 - 15
Main Authors:	Yang, Shenglong, Fang, Guonan, Zhang, Anpeng, Ruan, Banpu, Jiang, Hongzhen, Ding, Shilin, Liu, Chaolei, Zhang, Yu, Jaha, Noushin, Hu, Peng, Xu, Zhengjin, Gao, Zhenyu, Wang, Jiayu, Qian, Qian
Format:	Journal Article
Language:	English
Published:	England BioMed Central Ltd 26-08-2020 BioMed Central BMC
Subjects:	Aging - genetics Aging - physiology Apoptosis Catalase China Chlorophyll Chloroplasts Chromosome 2 Chromosomes Complementation Cultivars Deoxyribonucleic acid DNA DNA sequencing Early senescence Environmental degradation es2 Es2 gene Ethyl methanesulfonate Gene Expression Regulation, Plant Genes Genes, Plant Genotype & phenotype Inositol Inositol - genetics Inositol - metabolism Inositol polyphosphate Inositol polyphosphate kinase Kinases Leaves Mediation Membranes Metabolism Metabolites Missense mutation Molecular modelling Mutagenesis Mutants Mutation Nuclei (cytology) Oryza - genetics Oryza - physiology OsIPK2 Phenotypes Phosphotransferases (Phosphate Group Acceptor) - genetics Phosphotransferases (Phosphate Group Acceptor) - metabolism Photosynthesis Physiology Plant biochemistry Plant growth Plant Leaves - genetics Plant Leaves - physiology Plants Polyphosphate kinase Reactive oxygen species Rice Rice (Oryza sativa L.) Seedlings Senescence Signal transduction Transcription factors China Rice (Oryza sativa L.) Early senescence Inositol polyphosphate kinase es2 OsIPK2
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Summary:	Early leaf senescence influences yield and yield quality by affecting plant growth and development. A series of leaf senescence-associated molecular mechanisms have been reported in rice. However, the complex genetic regulatory networks that control leaf senescence need to be elucidated. In this study, an early senescence 2 (es2) mutant was obtained from ethyl methanesulfonate mutagenesis (EMS)-induced mutational library for the Japonica rice cultivar Wuyugeng 7 (WYG7). Leaves of es2 showed early senescence at the seedling stage and became severe at the tillering stage. The contents of reactive oxygen species (ROS) significantly increased, while chlorophyll content, photosynthetic rate, catalase (CAT) activity significantly decreased in the es2 mutant. Moreover, genes which related to senescence, ROS and chlorophyll degradation were up-regulated, while those associated with photosynthesis and chlorophyll synthesis were down-regulated in es2 mutant compared to WYG7. The ES2 gene, which encodes an inositol polyphosphate kinase (OsIPK2), was fine mapped to a 116.73-kb region on chromosome 2. DNA sequencing of ES2 in the mutant revealed a missense mutation, ES2 was localized to nucleus and plasma membrane of cells, and expressed in various tissues of rice. Complementation test and overexpression experiment confirmed that ES2 completely restored the normal phenotype, with chlorophyll contents and photosynthetic rate increased comparable with the wild type. These results reveal the new role of OsIPK2 in regulating leaf senescence in rice and therefore will provide additional genetic evidence on the molecular mechanisms controlling early leaf senescence. The ES2 gene, encoding an inositol polyphosphate kinase localized in the nucleus and plasma membrane of cells, is essential for leaf senescence in rice. Further study of ES2 will facilitate the dissection of the genetic mechanisms underlying early leaf senescence and plant growth.
ISSN:	1471-2229 1471-2229
DOI:	10.1186/s12870-020-02610-1