Extraction of Nitrate from Grapevine Petiole and its Determination by Using UV-Spectrometric Screening Method: a Comparative Study

Extraction of Nitrate from Grapevine Petiole and its Determination by Using UV-Spectrometric Screening Method: a Comparative Study

Estimation of nitrate accumulation in grapevine plant tissue has a great significance in grape production. In this method, nitrate was extracted in water by using magnesium carbonate and calcium hydroxide in the ratio of 10 : 1 and charcoal by agitating for 15 min, which gives clear, transparent, an...

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Bibliographic Details
Published in:Journal of analytical chemistry (New York, N.Y.) Vol. 78; no. 3; pp. 330 - 336
Main Authors: Patil, Rajendra S., Nandre, Vinod S., Mhetre, Sagar N., Mhetre, Narayan B., Sutar, Abhishek P., Jadhav, Kishor K., Mahajan, Dnyaneshwar M., Hase, Chandrakala P., Kamble, Pramod N.
Format: Journal Article
Language:English
Published: Moscow Pleiades Publishing 01-03-2023
Springer
Springer Nature B.V
Subjects:
Analysis
Analytical Chemistry
Calcium carbonate
Charcoal
Chemistry
Chemistry and Materials Science
Comparative analysis
Comparative studies
Dissolved organic carbon
Extraction (Chemistry)
Grapes
Grapevines
Hazardous wastes
Magnesium carbonate
Measurement
Methods
Nitrates
Pigments
Salicylic acid
Screening
Slaked lime
Spectra
Spectrometry
Spectrophotometry
Turbidity
Ultraviolet-visible spectrophotometry
India
nitrite
nitrate
carbonate
grapevine petiole
dissolved organic carbon
nitrate extraction
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Summary:Estimation of nitrate accumulation in grapevine plant tissue has a great significance in grape production. In this method, nitrate was extracted in water by using magnesium carbonate and calcium hydroxide in the ratio of 10 : 1 and charcoal by agitating for 15 min, which gives clear, transparent, and rapid extraction by removing pigments, color, and turbidity after filtration. Nitrate content in petiole sample extract was determined by a simple UV-spectrometric screening method at 220 nm wavelength which obeyed Beer’s law with linear behavior up to nitrate concentration of 11 ppm. The other species such as nitrite, carbonate, and dissolved organic carbon do not interfere. Spectrophotometric data on nitrate determination by other two methods, i.e., phenoldisulphonic acid and salicylic acid method, are included for comparison. The extraction and nitrate determination methods are innovative, reliable, easy to adapt, reduce time consumption and hazardous waste, moreover, they are suitable for routine laboratories where the sample load is very high.
ISSN:1061-9348
1608-3199
DOI:10.1134/S1061934823030115