Milk miRNA expression in buffaloes as a potential biomarker for mastitis

Milk miRNA expression in buffaloes as a potential biomarker for mastitis

Buffaloes have the highest potential for production due to a promising gene pool that is being enhanced and upgraded. Mastitis is a significant health impediment that greatly diminishes milk yield and quality, affecting rural farmers' livelihoods. The traditional gold standard used for diagnosi...

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Published in:BMC veterinary research Vol. 20; no. 1; p. 150
Main Authors: Jadhav, Abhishek B, Ingole, Shailesh D, Bharucha, Simin V, Yoshitha, Korsapati L, Gaikwad, Rajiv V, Pharande, Rajesh R, Kharde, Shambhudeo D
Format: Journal Article
Language:English
Published: England BioMed Central Ltd 20-04-2024
BioMed Central
BMC
Subjects:
Animals
Biomarkers
Bison
Buffalo milk
Buffaloes
Cattle
Cattle Diseases
Dairy industry
Diagnosis
Diseases
Female
Gene pool
Genetic aspects
Health aspects
Inflammatory diseases
Kinases
Laboratories
Mastitis
Mastitis, Bovine - diagnosis
Mastitis, Bovine - metabolism
MicroRNA
MicroRNAs
MicroRNAs - genetics
Milk
Milk - metabolism
Milk production
miRNA
Retrospective Studies
Thermal cycling
Tumorigenesis
Water buffalo
India
Lithuania
Mumbai India
miRNA
Mastitis
Buffaloes
Milk
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Summary:Buffaloes have the highest potential for production due to a promising gene pool that is being enhanced and upgraded. Mastitis is a significant health impediment that greatly diminishes milk yield and quality, affecting rural farmers' livelihoods. The traditional gold standard used for diagnosing mastitis or subclinical mastitis is CMT, but it has the drawback of false positive or negative results. Subclinical mastitis, if not treated promptly, can lead to mammary tumors. To address the gap in early diagnosis of subclinical mastitis in CMT-negative milk of buffaloes, we performed a retrospective analysis and evaluated the milk miRNA expression profiles as potential biomarkers. Thirty buffalo milk samples based on clinical signs and CMT were divided into normal, subclinical, and clinical mastitis. SCC evaluation showed significant differences between the groups. The data analysis demonstrated that the elevation of miR-146a and miR-383 differed substantially between normal, subclinical, and clinical mastitis milk of buffaloes with 100% sensitivity and specificity. The relationship of SCC with miR-146a and miR-383 in normal/healthy and subclinical mastitis was positively correlated. The overexpression of miR-146a and miR-383 is associated with inflammation. It can be a valuable prognostic and most sensitive biomarker for early mastitis detection in buffaloes with SCC below 2 lakhs and CMT-ve, enhancing the accuracy of subclinical mastitis diagnosis.
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ISSN:1746-6148
1746-6148
DOI:10.1186/s12917-024-04002-1