A novel binary pesticidal protein from Chryseobacterium arthrosphaerae controls western corn rootworm by a different mode of action to existing commercial pesticidal proteins

The western corn rootworm (WCR) Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae) remains one of the economically most important pests of maize (Zea mays) due to its adaptive capabilities to pest management options. This includes the ability to develop resistance to some of the commercial p...

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Bibliographic Details
Published in:	PloS one Vol. 18; no. 2; p. e0267220
Main Authors:	Jabeur, Rania, Guyon, Virginie, Toth, Szabolcs, Pereira, Adriano E, Huynh, Man P, Selmani, Zakia, Boland, Erin, Bosio, Mickael, Beuf, Laurent, Clark, Pete, Vallenet, David, Achouak, Wafa, Audiffrin, Carine, Torney, François, Paul, Wyatt, Heulin, Thierry, Hibbard, Bruce E, Toepfer, Stefan, Sallaud, Christophe
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 17-02-2023 Public Library of Science (PLoS)
Subjects:	Agricultural production Amino acids Analysis Animals Bacillus thuringiensis - genetics Bacteria Bacterial Proteins - metabolism Binding sites Bioassays Biology and Life Sciences Chryseobacterium Chryseobacterium - metabolism Coleoptera - genetics Corn Diabrotica virgifera virgifera Diseases and pests Endotoxins - metabolism Environmental aspects Genetic aspects Genomes Growth Health aspects Hypotheses Insecticide Resistance Insecticides Larva - metabolism Larvae Life Sciences Medicine and Health Sciences Mode of action Peptides Pest control Pest Control, Biological Pesticides Pesticides - pharmacology Pests Plants, Genetically Modified - metabolism Protein binding Proteins Recombinant proteins Research and Analysis Methods Vegetal Biology Zea mays - genetics United States United States > US Europe
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Summary:	The western corn rootworm (WCR) Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae) remains one of the economically most important pests of maize (Zea mays) due to its adaptive capabilities to pest management options. This includes the ability to develop resistance to some of the commercial pesticidal proteins originating from different strains of Bacillus thuringiensis. Although urgently needed, the discovery of new, environmentally safe agents with new modes of action is a challenge. In this study we report the discovery of a new family of binary pesticidal proteins isolated from several Chryseobacterium species. These novel binary proteins, referred to as GDI0005A and GDI0006A, produced as recombinant proteins, prevent growth and increase mortality of WCR larvae, as does the bacteria. These effects were found both in susceptible and resistant WCR colonies to Cry3Bb1 and Cry34Ab1/Cry35Ab1 (reassigned Gpp34Ab1/Tpp35Ab1). This suggests GDI0005A and GDI0006A may not share the same binding sites as those commercially deployed proteins and thereby possess a new mode of action. This paves the way towards the development of novel biological or biotechnological management solutions urgently needed against rootworms.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Competing Interests: The authors have declared that no competing interests exist.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0267220