Fourier synthesis optical diffraction tomography for kilohertz rate volumetric imaging
Many biological and soft matter processes occur at high speeds in complex 3D environments, and developing imaging techniques capable of elucidating their dynamics is an outstanding experimental challenge. Here, we introduce Fourier Synthesis Optical Diffraction Tomography (FS-ODT), a novel approach...
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| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
28-09-2023
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Many biological and soft matter processes occur at high speeds in complex 3D
environments, and developing imaging techniques capable of elucidating their
dynamics is an outstanding experimental challenge. Here, we introduce Fourier
Synthesis Optical Diffraction Tomography (FS-ODT), a novel approach for
high-speed quantitative phase imaging capable of recording the 3D refractive
index at kilohertz rates. FS-ODT introduces new pattern generation and inverse
computational strategies that multiplex tens of illumination angles in a single
tomogram, dramatically increasing the volumetric imaging rate. We validate
FS-ODT performance by imaging samples of known composition and accurately
recovering the refractive index for increasing pattern complexity. We further
demonstrate the capabilities of FS-ODT for probing complex systems by studying
the hindered diffusion of colloids in solution and the motility of
single-cellular bacterial swimmers. We believe that FS-ODT is a promising
approach for unlocking challenging imaging regimes in biophysics and soft
matter that have been little explored, including understanding the physical
interactions of colloids and microswimmers with their viscous 3D environment
and the interplay between these stimuli and the molecular response of
biological systems. |
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| DOI: | 10.48550/arxiv.2309.16912 |