Structures of the Human Gene for the Protein Disulfide Isomerase-Related Polypeptide ERp60 and a Processed Gene and Assignment of These Genes to 15q15 and 1q21

Structures of the Human Gene for the Protein Disulfide Isomerase-Related Polypeptide ERp60 and a Processed Gene and Assignment of These Genes to 15q15 and 1q21

ERp60 (also known as ERp61 or GRP58) is an isoform of protein disulfide isomerase (PDI) that has two thioredoxin-like domainsaanda′ in positions corresponding to those of domainsaanda′ in the PDI polypeptide and shows a significant amino acid sequence similarity to PDI in almost all parts. We report...

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Vol. 42; no. 3; pp. 397 - 404
Main Authors: Koivunen, Peppi, Horelli-Kuitunen, Nina, Helaakoski, Tarja, Karvonen, Päivi, Jaakkola, Marko, Palotie, Aarno, Kivirikko, Kari I.
Format: Journal Article
Language:English
Published: San Diego, CA Elsevier Inc 15-06-1997
Elsevier
Subjects:
Amino Acid Sequence
Base Sequence
Biological and medical sciences
Chromosome Mapping
Chromosomes, Human, Pair 1
Chromosomes, Human, Pair 15
Cloning, Molecular
DNA
Exons
Fundamental and applied biological sciences. Psychology
Gene Expression
Genes. Genome
Heat-Shock Proteins - chemistry
Heat-Shock Proteins - genetics
Humans
Introns
Isomerases - chemistry
Isomerases - genetics
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Peptides - chemistry
Peptides - genetics
Protein Disulfide-Isomerases
Pseudogenes
Thioredoxins - genetics
Genetic mapping
Human
Pseudogene
Molecular form
Intramolecular oxidoreductases
Nucleotide sequence
Enzyme
Transcription promoter
Chromosome A1
Tissue specificity
Gene organization
Gene expression
Isomerases
Analog
Chromosome D15
Protein disulfide-isomerase
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Summary:ERp60 (also known as ERp61 or GRP58) is an isoform of protein disulfide isomerase (PDI) that has two thioredoxin-like domainsaanda′ in positions corresponding to those of domainsaanda′ in the PDI polypeptide and shows a significant amino acid sequence similarity to PDI in almost all parts. We report here that the human ERp60 gene is about 18 kb in size and consists of 13 exons. No distinct correlation was found between its exon–intron organization and the modular structure of the ERp60 polypeptide, nor were any similarities in exon–intron organization found between the human ERp60, PDI, and thioredoxin genes. The 5′ flanking region of the ERp60 gene has no TATAA box or CCAAT motif but contains several potential binding sites for transcription factors. The highest levels of expression of the ERp60 mRNA were found by Northern blotting in the liver, placenta, lung, pancreas, and kidney, and the lowest in the heart, skeletal muscle, and brain. We also isolated an intronless ERp60 gene that probably represents a pseudogene. The ERp60 gene was mapped by fluorescencein situhybridization to 15q15 and the processed gene to 1q21, so that neither was located on the same chromosome as the human PDI and thioredoxin genes.
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ISSN:0888-7543
1089-8646
DOI:10.1006/geno.1997.4750